高效液相色谱法同时测定舒肝散中的绿原酸、阿魏酸和丹皮酚

Simultaneous Determination of Chlorogenic Acid, Ferulic Acid andPaeonol in Shugan Powder by HPLC

(目的)建立高效液相色谱(HPLC)法同时测定舒肝散中绿原酸、阿魏酸和丹皮酚的含量。(方法)用HPLC法测定舒肝散中绿原酸、阿魏酸和丹皮酚的含量。色谱条件为:资生堂MGⅡ色谱柱(∅4.6 mm×250 mm,5μm),流动相为乙腈(A)-水(B),流速为1.0 mL/min,检测波长为230 nm,柱温为30℃,进样量为10μL。(结果)舒肝散中绿原酸、阿魏酸和丹皮酚的质量浓度分别在0.994~19.890、0.970~19.00、2.538~50.75μg/m内线性关系良好,r分别为0.9997、0.9999、0.9998;平均加样回收率分别为100.14%、100.21%和98.83%。(结论)方法准确、简易、高效,可用于舒肝散中绿原酸、阿魏酸和丹皮酚含量的测定。

(Objective)To establish a HPLC method for simultaneous determination of chlorogenic acid,ferulic acid and paeonol in Shugansan powder.(Methods)The contents of chlorogenic acid,ferulic acid and paeonol in drugs were determined by HPLC.Chromatographic conditions:chromatographic column:Shiseido MGⅡ(∅4.6 mm×250 mm,5μm);Mobile phase:acetonitrile(A)-water(B),flow rate:1.0 mL/min;Detection wavelength:230 nm;Column temperature:30℃;Injection volume:10μL;(Results)The content of chlorogenic acid,ferulic acid and paeonol in Shugansan powder showed a good linear relationship with the peak area in the range of 0.994-19.890、0.970-19.00,2.538-50.75μg/mL,with r being 0.9997,0.9999 and 0.9998,respectively.The average recoveries were 100.14%,100.21%and 98.83%,respectively.(Conclusion)The method is accurate,simple and efficient,and can be used for the determination of chlorogenic acid,ferulic acid and paeonol in Shugans powder.