响应面法优化沙棘叶中总黄酮提取工艺

Optimization of total flavonoids extraction process from Hippophae rhamnoides L.leaves based on response surface methodology

目的通过Box-Behnken设计-响应面法确定超声波辅助提取中国沙棘叶总黄酮的最佳工艺参数,并探究其体外抗氧化活性。方法以中国沙棘叶为原料,通过单因素实验(料液比、乙醇浓度和超声时间)及响应面实验优化其黄酮提取工艺。结果最佳提取工艺条件为:料液比1:14(g:mL),乙醇浓度69%,超声时间19 min。在此条件下,黄酮提取量最高,为3.876 mg RE/g。所得沙棘提取液的总酚含量为4.457 mg GAE/g,并且具有较强的1,1-二苯基-2-三硝基苯肼(1,1-diphenyl-2-picrylhydrazyl,DPPH)自由基清除能力和铁离子还原能力(ferric ion reducing antioxidant power,FRAP)。结论优选出的工艺稳定可行,且具有良好的体外抗氧化活性,为中国沙棘叶的开发利用提供一定的理论依据。

Objective To determine the optimal process parameters for ultrasound assisted extraction of total flavonoids from Chinese Hippophae rhamnoides L.leaves using Box-Behnken design response surface methodology,and investigate its in vitro antioxidant activity.Methods The flavonoid extraction process of Chinese Hippophae rhamnoides L.leaves was optimized by one-way test(solid-liquid ratio,ethanol concentration and ultrasound time)and response surface test.Results The results showed that the suitable process parameters for extracting flavonoids from the leaves of the Hippophae rhamnoides L.were determined as follows:Solid-liquid ratio of 1:14(g:mL),ethanol concentration of 69%,and ultrasound time of 19 min.Under these process conditions,the maximum amount of flavonoids extracted was reached at 3.876 mg RE/g.In addition,the total phenolic content of the ultrasound-assisted extracted Hippophae rhamnoides L.leaves was 4.457 mg GAE/g and showed strong 1,1-diphenyl-2-picrylhydrazyl(DPPH)radical scavenging capacity and ferric ion reducing antioxidant power(FRAP).Conclusion The preferred process is stable and feasible,and the extract has good in vitro antioxidant activity,which provides certain theoretical basis for the development and utilization of Hippophae rhamnoides L.leaves in China.