反溶剂结合冷却结晶法纯化姜黄素的工艺研究

Purification Process of Curcumin by Antisolvent Combined Cooling Crystallization

目的:以姜黄根茎中提取纯化得到姜黄素粗品为原料,通过反溶剂结合冷却结晶法制备高纯度姜黄素。方法:采用冷却结晶法进行重结晶,丙酮为冷却结晶试剂,将反溶剂乙腈加入姜黄素丙酮溶液中。通过研究搅拌方式、冷却速率和反溶剂体积比对产物的影响,确定最佳工艺。结果:在机械搅拌和5℃/h冷却速率的条件下,使用丙酮-乙腈1:1.5(v:v)的溶剂冷却结晶,可得到较高纯度的姜黄素晶体,其收率高达97.30%,纯度为87.42%。结论:开发并优化了一种姜黄素结晶的制备工艺,实现了姜黄素产品质量的显著提升,为工业生产和科学研究提供了可靠的技术支持。

Objective:Crude curcumin was extracted and purified from the rhizome of curcuma longa,and high-purity curcumin was prepared by anti-solvent combined cooling crystallization.Methods:Recrystalization was carried out by cooling crystallization method,acetone was used as cooling crystallization reagent,and anti-solvent acetonitrile was added to curcumin in acetone solution.By studying the effects of stirring mode,cooling rate and antisolvent volume ratio on the product,the optimal process conditions were determined.Results:Under the condition of mechanical agitation and cooling rate of 5℃/h,using the solvent of acetone-acetonitrile 1:1.5(v:v)to cool and crystallize,high purity of curcumin crystals could be obtained,with a yield of 97.30%and a purity of 87.42%.Conclusions:A preparation process of curcumin crystals was developed and optimized,and the quality of curcumin products was successfully improved,which provided reliable technical support for industrial production and scientific research.