P16、Ki-67诊断宫颈鳞状上皮内病变价值及与高危型HPV感染关系

Value of P16 and Ki-67 detections for diagnosing cervical squamous intraepithelial lesions and the correlation between the P16 and Ki-67 detections and human papillomavirus infection

目的:研究P16、Ki-67诊断宫颈鳞状上皮内病变价值及与高危型人乳头瘤病毒(HPV)感染关系。方法:选取2022年2月-2024年2月本院收治的确诊宫颈鳞状上皮内病变患者150例纳入病例组,同期体检健康女性150例纳入健康组,以阴道镜检查和组织病理学检查结果作为金标准,采用液基薄层细胞检测(TCT)、HPV DNA检测、测定P16、Ki-67阳性表达情况,绘制受试者工作特征(ROC)曲线分析P16、Ki-67对宫颈鳞状上皮内病变的诊断价值;Spearman相关系数分析病例组P16、Ki-67阳性表达与HPV感染相关性。结果:病例组P16阳性表达(94.7%)、Ki-67阳性表达(93.3%)均高于对照组(3.3%、5.3%)(P<0.05);P16、Ki-67检出宫颈鳞状上皮内病变的准确率分别为;ROC曲线分析P16、Ki-67诊断宫颈鳞状上皮内病变的曲线下面积分别为0.957、0.940,敏感度94.7%、93.3%,特异度96.7%、94.7%;宫颈鳞状上皮内病变患者P16阳性表达、Ki-67阳性表达与HPV感染均呈正相关(r=0.705、0.726,均P<0.05)。结论:宫颈鳞状上皮内病变患者P16、Ki-67阳性率均异常升高,且阳性率与HPV感染呈正相关,对宫颈鳞状上皮内病变诊断价值较高。

Objective:To investigate the value of P16and Ki-67detections of patients for diagnosing their cervical squamous intraepithelial lesions,and to study the correlation between the P16and Ki-67 detections of the patients and their human papillomavirus(HPV)infection.Methods:150 women diagnosed with cervical squamous intraepithelial lesions according to the results of the colposcopy and the histopathological examination as the gold standard who treated in the hospital were selected in study group,and 150 healthy women were selected in control group from February 2022 to February 2024.The positive expressions of P16 and Ki-67 of the women were detected by Thinprep cytologic test(TCT)and the HPV DNA.Receiver operating characteristic(ROC)curve was drawn to analyze the diagnostic values of P16 and Ki-67 of the women for their cervical squamous intraepithelial lesions.Spearman correlation coefficient was used to analyze the correlation between the P16,Ki-67 positive expressions of the women and their HPV infection.Results:The positive expression rates of P16(94.7%)and Ki-67(93.3%)of the women in the study group were significantly higher than those(3.3%and 5.3%)of the women in the control group(P<0.05).ROC curve analysis showed that the area under the curve of the P16and Ki-67 positive expressions of the women for diagnosing their cervical squamous intraepithelial lesions were 0.957 and 0.940,the sensitivity of which were 94.7% and 93.3%,and the specificity of which were 96.7% and 94.7%.The positive expressions of the P16 and Ki-67 positive expressions of the women with cervical squamous intraepithelial lesions were positively correlated with their HPV infection(r=0.705,0.726,all P<0.05).Conclusion:The positive expressions of P16and Ki-67 of the women with cervical squamous intraepithelial lesions increase abnormally,and which are positively correlated with the HPV infection of the women,and both of them have the higher diagnostic value for the cervical squamous intraepithelial lesions of the women.