陕西省植物新记录--武陵山鳞毛蕨

Newly Recorded Species in Shaanxi Province-Dryopteris wulingshanensis

为进一步明确陕西省蕨类植物种类及分布,在陕西省汉中地区林草种质资源普查过程中,对调查到的植物进行标本采集和照片拍摄,通过形态学鉴定和系统发育分析,确定其为陕西省新记录植物。描述了其形态特征,并提供了标本及特征照片。通过DNA高通量测序,测定rbcL序列,采用最大似然法构建的系统发育树表明该物种与武陵山鳞毛蕨(Dryopteris wulingshanensis)的序列聚为一个分支。分子系统学研究结果与形态学鉴定结果一致,结合形态学和系统发育分析,确定此物种为陕西省植物新记录-武陵山鳞毛蕨。同时发现该种与吉首鳞毛蕨(Dryopteris jishouensis)的亲缘关系较近,可从叶柄基部维管束、叶形、叶片长宽比等方面对二者进行区分。此次武陵山鳞毛蕨天然居群为陕西省首次发现,扩大了武陵山鳞毛蕨的已知分布范围,丰富了陕西省物种资源多样性,为陕西省的植物区系补充了新的资料。凭证标本现存于山东省林草种质资源中心标本馆(SDF)。

To further clarify the species and distribution of ferns in Shaanxi province,a survey of forest and grass germplasm resources in Hanzhong area of Shaanxi Province was conductedby specimen collection and photo shoot Through morphological identification and phylogenetic analysis,it was proved to be a newly recorded specie in Shaanxi Province.The morphological characteristics were described,photos of the specimens and characteristics were provided.Through high-throughput DNA sequencing,the RuBisCO Large subunit(ITS)sequences were determined,and phylogenetic trees based on maximum likelihood method showed the sequences of this specie clustered into a branch with Dryopteris wulingshanensis,and the result of molecular systematics were consistent with the morphological identification result.Combined with morphology and phylogenetic analysis,this species was indentified as a new record in Shaanxi province-Dryopteris wulingshanensis.Meanwhile,the result also showed that the phylogenetic relationship with Dryopteris jishouensis was relatively close,the two species could be distinguished from aspects such as the vascular bundle at the base of the petiole,leaf shape,and leaf aspect ratio.The natural population of this plant was discovered for the first time in Shaanxi Province,which expanded the known distribution range of Dryopteris wulingshanensis,enriched the diversity of species resources in Shaanxi Province,and added new information to the flora of Shaanxi Province.The voucher specimen sare deposited in Herbarium of Shandong provincial Center of Forest and Grass Germplasm Resources(SDF).