固齿散对慢性牙周炎患者的效果及对龈沟液菌群多样性的影响

Effects of Guchi Powder on patients with chronic periodontitis and its influence on microbiota diversity of gingival crevicular fluid

目的探讨固齿散对慢性牙周炎患者的效果及对龈沟液菌群多样性的影响。方法选择邯郸市口腔医院2022年1月至2023年8月收治的112例慢性牙周炎患者为研究对象。按照随机数字表法将其分成观察组和对照组,各56例。对照组予以常规治疗,观察组在对照组基础上予以固齿散(0.5 g/次,2次/d)治疗,两组共治疗3个月。对比两组临床疗效,中医证候积分,牙周探诊相关指标情况[龈沟出血指数(sulcus bleeding index,SBI)、菌斑指数(plague index,PLI)及牙龈指数(gingival index,GI)],龈沟炎症因子[白细胞介素(interleukin,IL)-6、IL-1β],龈沟液菌群α多样性(Shannon指数、Simpson指数、Chao1指数、ACE指数)及β多样性。结果治疗后,观察组(92.86%)临床总有效率高于对照组(78.57%)(P<0.05);与对照组相比,观察组中医证候积分、SBI、PLI、GI、IL-6、IL-1β、Shannon指数降低(P<0.05,P<0.01),Simpson指数升高(P<0.05)。PC1对于样本差异的贡献值为70.90%,PC2对于样本差异的贡献值为24.10%。治疗后观察组与对照组整体距离存在一定分离趋势(P<0.05)。结论固齿散可调节慢性牙周炎患者龈沟液菌群多样性,改善牙周状况,减轻龈沟液炎症水平,效果显著。

Objective To investigate the effects of Guchi Powder on patients with chronic periodontitis and its influence on the microbiota diversity of gingival crevicular fluid(GCF).Methods A total of 112 patients with chronic periodontitis treated in Handan Stomatological Hospital from January 2022 to August 2023 were selected as the study subjects.They were divided into observation group and control group according to random number table method,with 56 cases in each group.The control group was given conventional treatment,while the observation group was administered Guchi Powder(0.5 g/dose,2 doses/d)on the basis of the control group for a total of three months.The clinical efficacy,TCM pattern score,periodontal probing-related indicators[sulcus bleeding index(SBI),plague index(PLI),and gingival index(GI)],gingival sulcus inflammatory factors[interleukin(IL)-6,IL-1β],microbiotaαdiversity in GCF[Shannon index,Simpson index,Chao1 index,and ACE index],andβdiversity were compared between the two groups.Results After treatment,the total clinical effective rate of observation group(92.86%)was higher than that of control group(78.57%)(P<0.05).Compared with the control group,the observation group showed decrease in TCM pattern scores,SBI,PLI,GI,IL-6,IL-1β,and Shannon index(P<0.05,P<0.01),while the Simpson index of GCF in the observation group increased(P<0.05).The contribution values of PC1 and PC2 to sample difference were 70.90% and 24.10%,respectively.After treatment,there was a certain separation trend in the overall distance between observation group and control group(P<0.05).Conclusion Guchi Powder can regulate the microbiota diversity of GCF in patients with chronic periodontitis,improve the periodontal condition,and reduce GCF inflammation level,with notable effects.