血清LncRNA MALAT1、miR-146a表达水平与炎症性肠病患者Th1/Th2、Th17/Treg免疫细胞平衡的关系研究

Study on the relationship between the expression levels of serum LncRNA MALAT1 and miR-146a and the balance of Th1/Th2 and Th17/Treg immune cells in patients with inflammatory bowel disease

目的探究血清长链非编码核糖核酸肺癌转移相关转录本1(LncRNA MALAT1)、微小核糖核酸-146a(miR-146a)表达水平与炎症性肠病(IBD)患者辅助性T细胞(Th)1/Th2、辅助性T细胞17(Th17)/调节性T细胞(Treg)免疫细胞平衡的关系。方法选取2021年6月至2023年6月我院诊治的IBD患者纳入观察组(n=151),根据疾病活动性将患者分为活动期组(n=78)和缓解期组(n=73),另选同期我院健康体检者纳入对照组(n=102)。采用实时荧光定量聚合酶链反应(QRT-PCR)法检测血清LncRNA MALAT1、miR-146a表达水平,流式细胞术检测外周血Th1、Th2、Th17、Treg细胞比例,并计算Th1/Th2比值、Th17/Treg比值,采用酶联免疫吸附法(ELISA)检测Th1/Th2、Th17/Treg相关细胞因子[白介素(IL)-6、肿瘤坏死因子-β(TNF-β)、干扰素-γ(IFN-γ)、白介素(IL)-10、白介素(IL)-17、白介素(IL)-22]。采用Pearson相关性分析LncRNA MALAT1、miR-146a表达水平与IBD患者Th1/Th2、Th17/Treg免疫细胞平衡的关系。结果三组血清LncRNA MALAT1、miR-146a表达水平、Th1、Th1/Th2、IFN-γ、IL-6、Th17、Th17/Treg、IL-17、IL-22比较,活动期组>缓解期组>对照组(P<0.05);三组Th2、TNF-β、Treg、IL-10比较,活动期组<缓解期组<对照组(P<0.05)。Pearson相关性分析显示:血清LncRNA MALAT1、miR-146a表达水平与Th1、Th1/Th2、IFN-γ、IL-6、Th17、Th17/Treg、IL-17、IL-22呈正相关(P<0.05),与Th2、TNF-β、Treg、IL-10呈负相关(P<0.05)。结论血清LncRNA MALAT1、miR-146a在IBD活动期患者中显著上调,且表达水平与Th1/Th2、Th17/Treg免疫细胞平衡密切相关,提示其可能通过调控免疫平衡影响IBD发生发展。

Objective To explore the relationship between the expression levels of serum long-chain non-coding ribonucleic acid lung cancer metastasis-associated transcript 1(LncRNA MALAT1)and microRNA-146a(miR-146a)and the balance of helper T cell(Th)1/Th2 and helper T cell 17(Th17)/regulatory T cell(Treg)immune cells in patients with inflammatory bowel disease(IBD).Methods IBD patients diagnosed and treated in our hospital from June 2021 to June 2023 were selected as observation group(n=151),patients were divided into active group(n=78)and the remission group(n=73)according to the disease activity,healthy subjects in our hospital during the same period were selected as control group(n=102).The expression levels of serum LncRNA MALAT1 and miR-146a were detected by real-time fluorescence quantitative polymerase chain reaction(QRT-PCR).The proportions of Th1,Th2,Th17 and Treg cells in peripheral blood were detected by flow cytometry,and the ratios of Th1/Th2 and Th17/Treg were calculated,Th1/Th2 and Th17/Treg related cytokines[interleukin(IL)-6,tumor necrosis factor-β(TNF-β),interferon-γ(IFN-γ),interleukin(IL)-10,interleukin(IL)-17,interleukin(IL)-22]were detected by enzyme-linked immunosorbent assay(ELISA).The relationship between the expression levels of LncRNA MALAT1 and miR-146a and the balance of Th1/Th2 and Th17/Treg immune cells in IBD patients was analyzed by Pearson correlation analysis.Results The expression levels of serum LncRNA MALAT1,miR-146a,Th1,Th1/Th2,IFN-γ,IL-6,Th17,Th17/Treg,IL-17 and IL-22 in three groups were compared,active group>remission group>control group(P<0.05).Th2,TNF-β,Treg and IL-10 in three groups were compared,active group<remission group<control group(P<0.05).Pearson correlation analysis showed that,the expression levels of serum LncRNA MALAT1 and miR-146a were positively correlated with Th1,Th1/Th2,IFN-γ,IL-6,Th17,Th17/Treg,IL-17 and IL-22(P<0.05),and negatively correlated with Th2,TNF-β,Treg and IL-10(P<0.05).Conclusion Serum LncRNA MALAT1 and miR-146a are significantly up-regulate in patients with active IBD,and the expression levels are closely relate to the balance of Th1/Th2 and Th17/Treg immune cells,suggesting that they may affect the occurrence and development of IBD by regulating immune balance.