摘要
为了鉴定拮抗番茄早疫病菌(Alternaria solani)的海洋细菌并评价其生防效果,本研究以前期从海洋中分离筛选的番茄早疫病菌拮抗细菌221-11为研究对象,根据形态学特征、生理生化特性和16S rDNA序列分析鉴定拮抗细菌的种类,再采用室内盆栽试验检测海洋拮抗细菌对番茄植株的促生效果以及对番茄早疫病的预防、治疗效果,最后测定拮抗菌对植物防御酶(SOD、POD、CAT)活性的影响.结果表明,海洋拮抗细菌221-11为贝莱斯芽孢杆菌(Bacillus velezensis),该菌对番茄早疫病菌抑菌圈直径为61.20±0.30 mm,抑制率为77.2%,且能抑制孢子萌发并致畸;盆栽灌根接种35 d发现5.75×10^(2)~5.75×10~^(8) CFU/mL海洋拮抗细菌221-11发酵液均能促进番茄植株生长,其中,以5.75×10^(8) CFU/mL发酵液处理株高、鲜质量和干质量最高,分别增长15.23%、64.23%和118.97%;发酵液的预防和治疗效果分别为63.45%和45.74%;接种高浓度221-11发酵液后叶片防御酶SOD、POD、CAT的活性显著增加,最高增加139.82%.综上所述,海洋拮抗细菌221-11对番茄植株有一定的促生潜力且对番茄早疫病有很好的防治效果,是生物防治番茄早疫病的潜在菌株.
In order to identify the marine bacteria antagonistic to tomato early blight(Alternaria solani)and evaluate its biocontrol effect,in this experiment,the species of the antagonistic bacterium 221-11 for tomato early blight screened in previous work was used as research object.Based on the morphological characteristics,physiological and biochemical properties,and 16S rDNA sequence analysis,the species of antagonistic bacterium was identified.Then,an indoor pot test was used to detect the growth-promoting effects of marine biocontrol bacteria on tomato plants and preventive,therapeutic effect on tomato early blight.Finally,the effects of antagonistic bacteria on the activities of plant defense enzymes(SOD,POD,CAT)were determined by using the aziridinium blue tetrazolium method,the guaiacol method,and the UV-absorbance method.The results showed that the marine antagonistic bacterium 221-11 was Bacillus velezensis.It produced an inhibition zone of 61.2±0.3 mm in diameter with 77.2%of inhibition rate against tomato early blight pathogen,and inhibited the spore germination and teratogenesis.After potting root inoculation for 35 d,it was found that 5.75×10^(2)-5.75×10^(8)CFU/mL of marine bacterium 221-11 fermentation solution were able to promote the growth of tomato plants.Among them,the plant height,fresh weight and dry weight of the plants treated with 5.75×10^(8) CFU/mL solution was the highest,which increased by 15.23%,64.23%and 118.97%,respectively.The preventive and therapeutic effects of the fermentation solution were 63.45%and 45.74%.Compared with the control group,the activities of SOD,POD and CAT after inoculation with biocontrol bacterium 221-11 increased with the increase of fermentation liquid concentration,and the highest increase was 139.82%.In summary,the biocontrol bacterium 221-11 has good control effect on tomato early blight and promotion potential to tomato plants,and is a potential strain for biological control of tomato early blight.
作者
杨星月
陆跃岭
金东林
王淑芳
焦豫良
杭新楠
YANG Xingyue;LU Yuelin;JIN Donglin;WANG Shufang;JIAO Yuliang;HANG Xinnan(College of Marine Science and Fisheries,Jiangsu Ocean University,Lianyungang Jiangsu 222005,China;Jiangsu Marine Biological Industry Technology Collaborative Innovation Center,Lianyungang Jiangsu 222005,China;Key Laboratory of Coastal Salt Marsh Ecosystems and Resources,Ministry of Natural Resources,Nanjing 210001,China)
出处
《植物医学》
2024年第5期18-27,共10页
Plant Health and Medicine
基金
江苏高校优势学科建设工程项目
江苏海洋大学研究生科研与实践创新计划项目(KYCX2022-66)。
关键词
番茄早疫病
拮抗细菌
贝莱斯芽孢杆菌
防治效果
防御酶
tomato early blight
antibacterial
Bacillus velezensis
control efficiency
defensive enzyme