咪鲜胺及其代谢物三氯苯酚在百合植株中的残留检测方法及其动态研究

Residue Determination and Dynamic Research of Prochloraz andIts Metabolite 2, 4, 6-Trichlorophenol in Lily Plants

建立了QuEChERS前处理结合高效液相色谱-串联质谱(HPLC-MS/MS)的方法检测百合植株中咪鲜胺的残留量.同时,采用气相色谱(GC)对咪鲜胺的主要代谢产物三氯苯酚在百合植株中的残留情况进行了测定和分析.结果表明,添加量为0.01~0.5 mg/kg时,咪鲜胺和三氯苯酚在百合植株中的平均回收率分别为80.2%~87.8%和81.1%~105.5%, RSD分别为5.2%~6.7%和3.0%~8.4%.消解动态结果表明,咪鲜胺降解速度快、残留低,属于容易降解的农药,但施用后咪鲜胺很快转化为毒性更强的环境污染物2, 4, 6-三氯苯酚,这为咪鲜胺在百合上的安全使用提供了参考数据.

A QuEChERS combined with high-performance liquid chromatography-tandem mass spectrometry(HPLC-MS/MS)and gas chromatography(GC)were established to detect the residues of prochloraz and its metabolite trichlorophenol in lily plants,respectively.The sample was extracted with acetonitrile,purified by dispersed solid-phase extraction,and detected by HPLC-MS/MS and GC.The results showed that at the spike levels of 0.01~0.5 mg/kg,the average recovery rates of prochloraz and trichlorophenol in lily plants were 80.2%~87.8%and 81.1%~105.5%with RSD of 5.2%~6.7%and 3.0%~8.4%,respectively.The analysis method is simple and accurate.The degradation dynamics of prochloraz in lily plants indicate that prochloraz has a fast degradation rate and low residue,which is an easily degradable pesticide.However,after application,prochloraz quickly transformed into the more toxic environmental pollutant 2,4,6-trichlorophenol.Results of this study provide the reference data for the safe use of prochloraz in lily.