转录组与代谢组联合分析三蕊柳对柳蓝叶甲的抗虫机制

Combined analysis of transcriptome and metabolome on the insect resistance mechanism of Salix triandra against the Plagiodera versicolora

为研究三蕊柳抗柳蓝叶甲的机制及其中的原理,以垂柳、黄花柳、旱柳为代表的非抗虫组和以3个无性系的三蕊柳为代表的抗虫组4种不同且相关的柳树品种为试验对象,以差异代谢物儿茶素溶液、水杨酸溶液、没食子酰葡萄糖溶液、新橙皮苷溶液以及异泽兰黄素溶液浸泡处理的叶片饲喂柳蓝叶甲成虫及幼虫进行试验,探究三蕊柳抗柳蓝叶甲的机制。结果表明:(1)柳蓝叶甲在食用异泽兰黄素溶液处理的叶片后出现死亡:24 h后成虫存活率平均为83.33%,48 h后成虫存活率平均为40%;24 h后幼虫存活率平均为80%,48 h后幼虫存活率平均为35%。(2)其他溶液处理的叶片均未造成柳蓝叶甲死亡:24 h后及48 h后,成虫、幼虫的成活率均为100%。(3)结合转录组与代谢组分析结果筛选出来的5种差异代谢物溶液处理叶片饲喂柳蓝叶甲之后,只有异泽兰黄素溶液处理出现柳蓝叶甲死亡,异泽兰黄素可能是三蕊柳抗柳蓝叶甲的重要原因。综上所述,推测三蕊柳抗虫机制与次生代谢物——异泽兰黄素有关。

To investigate the mechanism and the principle in the resistance to the Plagiodera versicolora in Salix triandra,Four different but related willow species were selected as the test materials,which included the insect-resistant group represented by three clones of S.triandra and three non-insect-resistant groups represented by S.babylonica,S.caprea and S.matsudana.The Plagiodera versicolora adults and larvae were fed with leaves treated with the differential metabolites Catechin solution,Salicylic acid solution,Gallic acid glucose solution,Neophesperidin solution and Eupatilin solution to explore the mechanism of resistance to the Plagiodera versicolora in Salix triandra.The results showed that:(1)The Plagiodera versicolora showed mortality after consuming leaves treated with Eupatilin solution:The average survival rate of adults was 83.33%after 24 h and 40%after 48 h.The average survival rate of larvae was 80%after 24 h and 35%after 48 h.(2)None of the leaves treated with other solutions caused death of Plagiodera versicolora:After 24 h and 48 h,the survival rate of adults and larvae was 100%without any mortality.(3)After feeding Plagiodera versicolora to leaves treated with the five differential metabolite solutions screened by combining the results of transcriptome and metabolome analyses,only the Eupatilin solution caused Plagiodera versicolora death.Eupatilin might be an important reason for the resistance of Salix triandra to Plagiodera versicolora.Therefore,it was speculated that the mechanism of insect resistance of S.triandra was related to the secondary metabolite-Eupatilin.