LncRNA UCA-1对肾细胞癌OS-RC-2细胞增殖、凋亡的影响及机制

Effects and Mechanisms of LncRNA UCA-1 on the Proliferation and Apoptosis of Renal Cell Carcinoma OS-RC-2 Cells

目的研究尿路上皮癌相关基因1(UCA-1)对肾细胞癌OS-RC-2细胞恶性生物学进程的影响。方法通过实时荧光定量PCR方法分析肾细胞癌组织与癌旁组织中UCA-1的表达;将肾细胞癌OS-RC-2细胞随机设置为3个实验组:siRNA NC组、siRNA UCA-1组与LY294002组。采用MTT实验方法分析OS-RC-2细胞的增殖速度;采用Transwell实验方法分析OS-RC-2细胞的侵袭数量;采用流式细胞术检测OS-RC-2细胞的凋亡情况;采用蛋白免疫印迹方法分析PI3K/AKT通路相关蛋白的表达。结果与肾细胞癌旁的组织比较,肾细胞癌组织的UCA-1表达上调。与siRNA NC组比较,siRNA UCA-1组以及LY294002组的OS-RC-2细胞增殖能力下降(P<0.05);OS-RC-2细胞侵袭数量下降(P<0.05);OS-RC-2细胞凋亡率增加(P<0.05);OS-RC-2细胞PI3K、AKT蛋白表达下调(P<0.05)。结论UCA-1在肾细胞癌细胞中表达增加,抑制UCA-1表达后,OS-RC-2细胞的增殖速度与侵袭数量降低,凋亡率增加,该机制与UCA-1调节PI3K/AKT信号通路相关。

Objective To investigate the effect of urothelial carcinoma associated gene 1(UCA-1)on the malignant biological process of renal cell carcinoma OS-RC-2 cells.Methods The expression of UCA-1 in renal cell carcinoma tissues and adjacent tissues was detected by real-time fluorescence quantitative PCR.Renal cell carcinoma OS-RC-2 cells were randomly divided into three experimental groups:siRNA NC group,siRNA UCA-1 group and LY294002 group.The proliferation rate of OS-RC-2 cells was analyzed by MTT assay.Transwell assay was used to analyze the invasion of OS-RC-2 cells.The apoptosis of OS-RC-2 cells was detected by flow cytometry.The expression of PI3K/AKT pathway related proteins was analyzed by Western blot.Results The expression of UCA-1 was up-regulated in renal cell carcinoma tissues compared with adjacent renal cell carcinoma tissues.Compared with those in the siRNA NC group,in the siRNA UCA-1 and the LY294002 group the proliferation ability of OS-RC-2 cells was decreased(P<0.05),the invasion number of OS-RC-2 cells was significantly decreased(P<0.05),the apoptosis rate of OS-RC-2 cells was increased(P<0.05),the expression levels of PI3K and AKT proteins in the OS-RC-2 cells were significantly decreased(P<0.05).Conclusion The expression of UCA-1 increases in renal cell carcinoma.After inhibiting UCA-1 expression,the proliferation rate and invasion number of OS-RC-2 cells decrease,and the apoptosis rate increases,which may be related to UCA-1 regulating the PI3K/AKT signaling pathway.