Robust reactive oxygen species modulator hitchhiking yeast microcapsules for colitis alleviation by trilogically intestinal microenvironment renovation

Ulcerative colitis(UC)is characterized by chronic inflammatory processes of the intestinal tract of unknown origin.Current treatments lack understanding on how to effectively alleviate oxidative stress,relieve inflammation,as well as modulate gut microbiota for maintaining intestinal homeostasis synchronously.In this study,a novel drug delivery system based on a metal polyphenol network(MPN)was constructed via metal coordination between epigallocatechin gallate(EGCG)and Fe^(3+).Curcumin(Cur),an active polyphenolic compound,with distinguished anti-inflammatory activity was assembled and encapsulated into MPN to generate Cur-MPN.The obtained Cur-MPN could serve as a robust reactive oxygen species modulator by efficiently scavenging superoxide radical(O_(2)•-)as well as hydroxyl radical(⋅OH).By hitchhiking yeast microcapsule(YM),Cur-MPN was then encapsulated into YM to obtain CM@YM.Our findings demonstrated that CM@YM was able to protect Cur-MPN to withstand the harsh gastrointestinal environment and enhance the targeting and retention abilities of the inflamed colon.When administered orally,CM@YM could alleviate DSS-induced colitis with protective and therapeutic effects by scavenging ROS,reducing pro-inflammatory cytokines,and regulating the polarization of macrophages to M1,thus restoring barrier function and maintaining intestinal homeostasis.Importantly,CM@YM also modulated the gut microbiome to a favorable state by improving bacterial diversity and transforming the compositional structure to an anti-inflammatory phenotype as well as increasing the content of short-chain fatty acids(SCFA)(such as acetic acid,propionic acid,and butyric acid).Collectively,with excellent biocompatibility,our findings indicate that synergistically regulating intestinal microenvironment will be a promising approach for UC.