PK型紫苏叶精油GC-MS成分分析及抗氧化活性研究

Study on the Component Analysis of Essential Oil from PK-type Perilla frutescens Leaves by GC-MS and Its Antioxidant Activity

为了深入探究PK型紫苏叶精油的抗氧化潜力,通过气相色谱分析(GC)技术筛选出一种紫苏酮含量高于40%的精油,进一步采用GC-MS技术对该精油的化学成分进行鉴定,并分析该精油的体外抗氧化活性,为PK型紫苏叶精油在抗氧化领域的开发利用提供科学依据。结果表明:紫苏叶精油中含量大于0.5%的化学成分有17种,均为萜类及其衍生物,占精油总含量的89.72%,其中含量较高的组分有紫苏酮(44.98%)、异白苏烯酮(13.06%)和β-石竹烯(10.53%)。体外抗氧化活性试验结果显示:PK型紫苏叶精油对DPPH和ABTS自由基都有清除能力,其IC50值分别为7.00、2.66 mg·mL^(−1),且具有一定的铁还原能力和总抗氧化能力,其RP0.5AU分别为11.96、1.83 mg·mL^(−1)。研究表明PK型紫苏叶精油的上述抗氧化活性指标均与浓度呈正相关。

In order to deeply investigate the antioxidant potential of essential oil from PK-type Perilla frutescens leaves,an essential oil with a content of perilla ketone higher than 40%was screened out by using the gas chromatographic analysis(GC)technology.Then,the chemical composition of the essential oil was further identified by using the GC-MS technology,and the antioxidant activity in vitro of the essential oil was analyzed,which provided a scientific basis for the development and utilization of the essential oil from PK-type Perilla frutescens leaves in the field of antioxidation.The results showed that there were 17 kinds of chemical components with the content of more than 0.5%in the essential oil from Perilla frutescens,all of which were terpenes and their derivatives,accounting for 89.72%of the total content of essential oil.The components with higher content were perilla ketone(44.98%),isoegomaketone(13.06%)andβ-caryophyllene(10.53%).The experimental results of antioxidant activity in vitro showed that:the essential oil from PK-type Perilla frutescens leaves had scavenging ability to both DPPH and ABTS radicals,with IC50 values of 7.00 and 2.66 mg·mL^(−1),respectively.It also had certain iron reduction ability and total antioxidant capacity,with the RP0.5 AU values of 11.96 and 1.83 mg·mL^(−1).It was shown that all the above antioxidant activity indexes of the essential oil from PK-type Perilla frutescens leaves were positively correlated with the concentration.