环状RNA hsa_circ_0003221靶向miR-139-3p/IGF2BP3轴调控口腔鳞癌细胞增殖和凋亡的分子机制

Molecular mechanism of Cyclic RNA hsa_circ_0003221 in regulating the proliferation and apoptosis of oral squamous cell carcinoma cells by targeting miR-139-3p/IGF2BP3 axis

目的:探讨环状RNA hsa_circ_0003221(circPTK2)靶向微小RNA(miR)-139-3p/胰岛素样生长因子2 mRNA结合蛋白3(IGF2BP3)轴调控口腔鳞癌(OSCC)细胞增殖、凋亡。方法:qRT-PCR检测组织及细胞中circPTK2、miR-139-3p、IGF2BP3 mRNA表达水平;将CAL-27细胞分为5组:sh circPTK2、sh NC、sh circPTK2+miR-139-3p inhibitor、sh circPTK2+inhibitor NC、空白组。分别检测细胞增殖率、凋亡率及相关蛋白、IGF2BP3的表达,并验证miR-139-3p与circPTK2、IGF2BP3的靶向关系。结果:miR-139-3p表达在OSCC组织及细胞中降低,circPTK2、IGF2BP3 mRNA表达增加(P<0.05);沉默circPTK2可抑制细胞增殖及circPTK2、IGF2BP3 mRNA及蛋白表达,促进细胞凋亡及miR-139-3p表达(P<0.05);miR-139-3p inhibitor逆转了沉默circPTK2对CAL-27细胞恶性行为的抑制作用(P<0.05)。miR-139-3p分别与circPTK2、IGF 2BP3存在靶向关系。结论:沉默circPTK2可通过miR-139-3p/IGF2BP3轴调控OSCC细胞增殖、凋亡。

Objective:To investigate the regulation of proliferation and apoptosis of oral squamous cell carcinoma(OSCC)cells by circular RNA hsa_circ_0003221(circPTK2)targeting microrNA(miR)-139-3p/IGF2BP3 axis.Methods:The mRNA expression of circPTK2,miR-139-3p and IGF2BP3 in tissues and cell lines was detected by qRT-PCR.OSCC CAL-27 cells were divided into 5 groups:sh circPTK2,sh NC,sh circPTK2+miR-139-3p inhibitor,sh circPTK2+inhibitor NC and blank control groups.Cell proliferation rate,apoptosis rate,expression of related proteins and IGF2BP3 were respectively detected,and the targeting relationship between miR-139-3p and circPTK2 and IGF2BP3 was respectively verified.Results:The expression of miR-139-3p was decreased in OSCC tissues and the OSCC cell lines,and the mRNA expression of circPTK2 and IGF2BP3 was increased(P<0.05).Silencing circPTK2 inhibited cell proliferation,decreased mRNA and protein expression of circPTK2 and IGF2BP3,and promoted cell apoptosis and miR-139-3p expression(P<0.05).miR-139-3p inhibitor reversed the inhibitory effect of silencing circPTK2 on the malignant behavior of CAL-27 cells(P<0.05).miR-139-3p has a targeting relationship with circPTK2 and IGF2BP3,respectively.Conclusion:Silencing circPTK2 can regulate OSCC cell proliferation and apoptosis through miR-139-3p/IGF2BP3 axis.