ADRB2、FCER2、IL-13及IL-4基因多态性与环境因素对儿童哮喘易感性交互影响

Interaction of the ADRB2 RS1042713,FCER2 RS28364072,IL-13 RS20541,and IL-4 RS2243250 gene polymorphism and environmental factors on the susceptibility to childhood asthma

目的探讨β2肾上腺素受体(ADRB2)rs1042713、免疫球蛋白E高亲和力受体2(FCER2)rs28364072、白细胞介素-13(IL-13)rs20541及IL-4 rs22432504种基因多态性、环境因素及其交互作用对重庆地区儿童哮喘发病的影响,旨在为儿童哮喘的早期诊断及有效防治提供科学依据。方法选取2020年11月至2022年9月就诊于该院儿科哮喘门诊的哮喘患儿88例作为病例组,同期于该院儿童保健科健康体检的非哮喘健康儿童80例作为对照组。收集2组研究对象的人口学资料,运用多重聚合酶链反应结合高通量测序技术检测目标基因。使用logistic回归分析环境因素对儿童哮喘的影响,并采用广义多因子降维法深入探究基因与环境之间的交互作用。结果蟑螂、宠物暴露是儿童哮喘发病的明显危险因素(优势比=2.791、3.055,95%可信区间1.353~5.757、1.453~6.423,P=0.005、0.003)。2组研究对象rs1042713、rs28364072、rs20541基因型分布比较,差异均有统计学意义(P<0.05);rs2243250基因型分布比较,差异无统计学意义(P>0.05)。rs2836072与rs20541、rs1042713位点的交互作用对儿童哮喘的发病具有明显影响,差异均有统计学意义(P<0.05)。结论重庆地区蟑螂、宠物暴露是增加儿童哮喘风险的重要因素,rs1042713、rs2836072、rs20541基因多态性与儿童哮喘的易感性密切相关,这些基因多态性与环境因素的交互作用共同影响着儿童哮喘的发病风险。

Objective To investigateβ2-adrenergic receptor(ADRB2)rs1042713,immunoglobulin E high affinity receptor 2(FCER2)rs28364072,interleukin-13(IL-13)rs20541 and interleukin-4(IL-4)rs2243250 gene polymorphisms,environmental factors and their interactions on the incidence of childhood asthma in Chongqing,aiming to provide scientific basis for the early diagnosis and effective prevention and treatment of childhood asthma.Methods A total of 88 children with asthma who visited the pediatric asthma clinic of the hospital from November 2020 to September 2022 were selected as the case group,and 80 non-asthmatic healthy children who underwent physical examination in the children′s health care department of the hospital during the same period were selected as the control group.The demographic data of the two groups were collected,and the target genes were detected by multiplex polymerase chain reaction(PCR)combined with high-throughput sequencing technology.Logistic regression was used to analyze the effect of environmental factors on childhood asthma,and generalized multifactor dimensionality reduction(GMDR)was used to deeply explore the interaction between genes and environment.Results Cockroach exposure(OR=2.791,95%CI 1.353-5.757,P=0.005)and pet exposure(OR=3.055,95%CI 1.453-6.423,P=0.003)were significant risk factors for asthma in children,and the differences were statistically significant.There were significant differences in the genotype distribution of rs1042713,rs28364072 and rs20541 between the two groups(P<0.05).There was no significant difference in the genotype distribution of rs2243250 between the two groups(P>0.05).There wa s no significant difference in the genotype distribution of rs2243250 between the two groups(P<0.05).Conclusion Cockroach and pet exposure are important factors that increase the risk of childhood asthma in Chongqing,and the rs1042713,rs2836072 and rs20541 gene polymorphisms are closely related to the susceptibility to childhood asthma.The interaction of these gene polymorphisms and environmental factors affects the risk of childhood asthma..