外源锌对气生铁兰抗寒生理特征的影响

Effect of Exogenous Zinc on Physiological Characteristics of Cold Resistance in Tillandsia

为研究外源锌对气生铁兰抗寒生理特征的影响,自然低温下,以6个品种为供试材料,设置0、100、150、200、250、300、350 mg/L 7个锌浓度处理,结果表明:随着外源锌浓度的升高,各品种叶绿素含量(Chl)、渗透调节物质含量(Pro)、抗氧化酶活性(SOD、POD)均呈先增加后减少的变化趋势,膜脂过氧化指标(MDA、REC)则相反;适宜浓度的外源锌(200~300 mg/L)可使低温胁迫下气生铁兰细胞膜的过氧化程度减轻,抗氧化能力增强,细胞内外的渗透压稳定,光合生理状态良好,植物生长健康,低温胁迫受到的伤害亦明显减弱,抗寒性明显提高,具体表现为Chl含量、PRO含量、抗氧化酶活性均较高,MDA含量和REC均较低。

To explore the effect of exogenous zinc on physiological characteristics of cold resistance in Tillandsia under natural low temperature,this study is based on six species of Tillandsia as experimental material with seven zinc treatments,respectively 0,100,150,200,250,300,350 mg/L.The results showed that with the increase of zinc concentration,the changes of chlorophyll content(Chl),proline content(Pro),activity of antioxidant enzymes(SOD and POD)all increased first and then decreased,the membrane lipid peroxidation index(MDA and REC)was opposite;when the concentration of zinc was 200-300 mg/L,the membrane peroxidation was reduced,the antioxidation ability of the cells was enhanced,the osmotic pressure was stable,the photosynthetic physiological state was good,the plant grew healthily,the damage caused by low temperature stress was also obviously weakened,and the cold resistance was significantly improved,such as the content of Chl,Pro and antioxidant enzyme activity were higher,the content of MDA and REC were lower.