尖叶茶藨子种子无菌苗再生体系的建立

Establishment of Rapid Propagation for Sterile Seedlings of Ribes maximowiczianum Komarov by Tissue Culture

以尖叶茶藨子成熟种子为试材,采用不同处理及消毒方式获得无菌苗,再以幼苗茎段为外植体,研究不同植物生长调节剂对丛芽诱导、增殖、壮苗及生根的影响。结果表明:500 mg/L GA_(3)浸泡处理有利于种子的萌发,发芽率可达66.7%,最佳消毒处理为75%乙醇30 s+0.1%HgCl_(2)消毒8 min,污染率为23.3%;最佳丛芽诱导培养基为MS+2.0 mg/L 6-BA+0.05 mg/L NAA,增殖倍数为7.5;最佳壮苗培养基为MS+0.1 mg/L 6-BA+0.05 mg/L NAA+50 g马铃薯泥;最佳生根培养基为1/2MS+2.0 mg/L IBA,生根率为100%,平均生根条数为6.33。炼苗后,移栽到泥炭土∶椰糠∶珍珠岩体积比为3∶1∶1的基质中,成活率达87.5%。该研究建立了尖叶茶藨子种子组培快速繁育技术体系,适用于尖叶茶藨子种苗的规模化生产和应用。

The seedlings of mature Ribes maximowiczianum Komarov seed germination under different disinfection treatment were used as explants to study the influence of different plant growth regulators on buds induction,hyperplasia,sound seedling and rhizogenesis.The results showed that 500 mg/L GA_(3)soaking treatment was conducive to seed germination,and the germination rate could reach 66.7%,and the best disinfection treatment was 75%alcohol for 30s and 0.1%mercuric acid disinfection for 8 min,the contamination rate was 23.3%.The best medium for bud induction was MS+2.0 mg/L 6-BA+0.05 mg/L NAA,the proliferation times was 7.5.The best medium for rooting was 1/2MS+2.0 mg/L IBA,the rooting rate was 100%,and the average number of rooting shoots was 6.33.The survival rate was 87.5%in the medium of 3∶1∶1 in peat soil∶coir∶perlite.This study established a complete system of tissue culture and rapid propagation for Ribes maximowiczianum Komarov seed,which is suitable for the large-scale production and application of Ribes maximowiczianum Komarov seedlings.