新型植物免疫激活蛋白维大力(VDAL)对烟草花叶病毒抑制作用

Inhibitory Effect of Novel Plant Immune Activating Protein VDAL on Tobacco Mosaic Virus

[目的]明确新型植物免疫激活蛋白维大力(VDAL)对烟草花叶病毒(TMV)的抑制作用。[方法]采用半叶枯斑法和分光光度法,分别测定植物免疫激活蛋白VDAL对TMV的抑制作用和烟草植株过氧化物酶(POD)活性的影响。[结果]VDAL对TMV具有较好的体外钝化作用,当钝化时间为90 min时,枯斑抑制率可达到80.76%;喷药3次后抑制率为85.04%,且过氧化物酶活性较对照增加了1.26倍。[结论]VDAL可能通过对病毒的钝化作用并诱导烟草植株产生防御反应,进而防治烟草花叶病毒病。

[Objective]To clarify the inhibitory effect of a new plant immune activating protein(VDAL)on tobacco mosaic virus(TMV).[Method]The inhibition of plant immune activating protein(VDAL)on TMV and the effect on peroxidase(POD)activity in tobacco plants were determined by spot inhibition and spectrophotometry.[Result]VDAL had a good inactivation effect on TMV in vitro.When the inactivation time was 90 min,the inhibition rate of lesions could reach 80.76%.After three times of spraying,the inhibition rate could rise to 85.04%,and the peroxidase activity increased 1.26 times compared with the control.[Conclusion]VDAL may control TMV disease by inactivating the virus and inducing the defense response of tobacco plants.