Box-Behnken响应面法优选低共熔溶剂提取巴西人参总皂苷工艺

Optimization of Deep Eutectic Solvents Extraction of Total Saponins from Pfaffia glomerata by Box-Behnken Response Surface Methodology

[目的]响应面法优选巴西人参总皂苷提取工艺,为其进一步开发利用提供参考。[方法]以苋科植物巴西人参为研究对象,采用超声辅助低共熔溶剂法提取总皂苷,通过单因素试验对尿素与氯化胆碱摩尔比、含水量、液料比、提取时间进行考察,以总皂苷得率作为评价指标,运用Box-Behnken响应面法对巴西人参总皂苷提取工艺进行优化。[结果]超声辅助低共熔溶剂提取巴西人参总皂苷的最佳工艺为尿素∶氯化胆碱=1∶1、含水量38%、液料比18∶1(mL∶g)、提取时间37 min,在此条件下,总皂苷得率为6.26%。[结论]该方法提取效率高、重复性好、操作简便,可用于巴西人参总皂苷的提取。

[Objective]Response surface methodology was used to optimize the extraction process of total saponins from Pfaffia glomerata,providing reference for its further development and utilization.[Method]Taking amaranthaceous plant Pfaffia glomerata as the research object,total saponins were extracted using ultrasound assisted deep eutectic solvent method.Through single factor experiments,the molar ratio of urea to choline chloride,water content,liquid-solid ratio and extraction time were investigated,the total saponin yield was used as the evaluation index,and the Box-Behnken response surface methodology was used to optimize the extraction process of total saponins from Pfaffia glomerata.[Result]The optimal process for ultrasound-assisted deep eutectic solvents extraction of total saponins from Pfaffia glomerata was established with the following parameters:urea to choline chloride ratio of 1∶1,water content of 38%,liquid-material ratio of 18∶1(mL∶g),and extraction time of 37 minutes.Under the conditions,the total saponin yield achieved was 6.26%.[Conclusion]This method has high extraction efficiency,good repeatability and simple operation,and can be used for the extraction of total saponins from Pfaffia glomerata.