萼翅藤多糖超声波辅助提取及抗氧化活性工艺的优化

Optimization of Ultrasonic Assisted Extraction and Antioxidant Activity of Polysaccharides from Calycopteris f loribunda

采用超声波辅助技术提取萼翅藤多糖成分,以多糖提取量为指标,通过单因素试验考察超声时间、料液比、超声功率、超声温度对萼翅藤多糖提取量的影响。在此基础上设计正交试验优化提取工艺,最后以·OH自由基和DPPH·自由基清除能力为指标评价萼翅藤多糖的抗氧化活性。结果表明,萼翅藤多糖最佳提取工艺条件为提取时间40 min,料液比1∶50 g/mL,超声功率360 W,提取温度15℃,在此条件下多糖平均提取量达到39.71 mg/g。萼翅藤多糖对·OH和DPPH·自由基的半数抑制浓度(IC_(50))分别为1.69mg/mL和10.45μg/mL,清除率分别达99.79%、91.45%。萼翅藤多糖成分具有良好的抗氧化活性,可作为潜在的抗氧化剂用于食品加工和医药研发领域研究。

Ultrasonic-assisted technology was used to extract polysaccharides from Calycopteris floribunda.The effects of ultrasonic time,solid-liquid ratio,ultrasonic power and ultrasonic temperature on the extraction amount of polysaccharides from Calycopteris floribunda were investigated by single factor test.On this basis,orthogonal experiments were designed to optimize the extraction process.Finally,the antioxidant activity of polysaccharides was evaluated using·OH free radical and DPPH·free radical scavenging ability as indexes.The results showed that under the optimal extraction conditions,the extraction time was 40 min,the solid-liquid ratio was 1∶50 g/mL,the ultrasonic power was 360 W,and the extraction temperature was 15℃.The average extraction amount of polysaccharides reached 39.71 mg/g.The median inhibitory concentration(IC_(50))of polysaccharides on·OH and DPPH·free radicals were 1.69 mg/mL and 10.45μg/mL,respectively,and the clearance rates were 99.79%and 91.45%,respectively.The polysaccharides of Calycopteris f loribunda had good antioxidant activity.It can be used as a potential antioxidant for food processing and pharmaceutical research and development.