间性猪垂体的全转录组学特征分析

Analysis of Whole Transcriptome Characteristics of the Hermaphroditic Pig’s Pituitary Gland

旨在探索间性猪垂体的编码与非编码RNAs的表达特征,为解析间性猪垂体功能紊乱的分子机制提供数据支持。以5月龄正常母猪和间性猪各3头为研究对象,进行血清激素检测和垂体组织的全转录组测序,分析鉴定间性猪垂体差异表达的mRNAs、lncRNAs、miRNAs,并构建间性猪垂体中相关基因调控的竞争性内源RNAs(ceRNAs)。结果表明,间性猪血清激素分泌紊乱,垂体功能异常。与正常母猪对比,间性猪垂体差异表达的mRNAs有1451个,差异表达的lncRNAs有277个,差异表达的miRNAs有17个。其中差异表达mRNAs主要富集在MAPK信号通路、孕酮介导的卵母细胞成熟、PRL信号通路等生物通路上;ceRNAs网络分析发现,TCONS_00175477-novel_265-CCNB3、TCONS_00134726-novel_265-ZNF366和TCONS_00212783-novel_265-ZNF366竞争组合可能与间性猪垂体激素分泌异常有关。综上所述,本研究揭示了间性猪垂体mRNAs、lncRNAs和miRNAs差异表达并构建ceRNAs,其特定lncRNA-miRNA-mRNA可能参与间性猪垂体的激素合成与分泌的调控,为解析间性猪垂体功能紊乱的分子机制提供了理论参考。

The aim of this study was to explore the expression characteristics of coding and non-coding RNAs in the pituitary gland of hermaphroditic pigs,thereby providing supporting data for the analysis of the molecular mechanisms underlying pituitary dysfunction in these pigs.Three five-month-old normal sows and three hermaphroditic pigs were used for the study.Serum hormone assays and whole transcriptome sequencing of pituitary tissues were carried out to analyze and identify differentially expressed mRNAs,lncRNAs,and miRNAs in the pituitary of hermaphroditic pigs.Additionally,a competing endogenous RNA(ceRNA)network was established to elucidate the regulatory relationships of related genes in the pituitary of hermaphroditic pigs.Disrupted serum hormone secretion and abnormal pituitary function were observed in hermaphroditic pigs.Comparison with normal sows,1451 differentially expressed mRNAs,277 differentially expressed lncRNAs,and 17 differentially expressed miRNAs were identified in the pituitary gland of hermaphroditic pigs,which were enriched in biological pathways,such as the MAPK signaling pathway,the progesterone-mediated oocyte maturation,and the PRL signaling pathway.Through the analysis of the ceRNA network,competitive combinations of TCONS_00175477-novel_265-CCNB 3,TCONS_00134726-novel_265-ZNF 366 and TCONS_00212783-novel_265-ZNF 366 were identified possibly related to abnormal pituitary hormone secretion in hermaphroditic pigs.In summary,the differentially expressed mRNAs,lncRNAs and miRNAs in the pituitary gland of hermaphroditic pigs were revealed,thereby ceRNA networks were constructed,which may be involved in regulating hormone synthesis and secretion in this study.All these were aid to support the elucidation of the molecular mechanisms underlying the dysfunction of the pituitary gland in hermaphroditic pigs.