橘小实蝇抗菌肽全基因组鉴定和表达模式分析

Genome-wide identification and expression-profiling analysis of the antimicrobial peptide genes in Bactrocera dorsalis(Hendel)

基于橘小实蝇染色体水平的基因组数据,运用生物信息学方法,深入分析橘小实蝇抗菌肽基因的组成、结构特征及它们在昆虫间的系统进化关系;使用荧光定量PCR技术,分析橘小实蝇抗菌肽基因在橘小实蝇4个不同发育阶段及成虫的6个部位的表达谱。研究结果表明,橘小实蝇基因组存在3大类共29个抗菌肽基因,包括12个cecropins,11个defensins,4个attacins和2个diptericins。基因组和蛋白结构表明橘小实蝇抗菌肽分子和结构具有显著的多样性,包括基因复制过程;表达谱分析表明,大多数抗菌肽在蛹期和成虫期表达水平较高,且在不同部位中,不同类型抗菌肽的表达特征差异较大;此外,除了在血淋巴中的广泛表达外,某些抗菌肽基因在头和体壁中也有特定表达。上述结果为进一步研究橘小实蝇抗菌肽的功能提供了数据库和信息。

The antimicrobial peptide(AMP)genes of Bactrocera dorsalis were identified at the genome level to explore potential AMPs for the control of B.dorsalis.The composition,structural features,and phylogenetic relationships of AMPs across different insect species were analyzed by using bioinformatics methods based on chromosome-level genomic data.Furthermore,expression profiles of AMPs at different developmental stages in six adult parts were examined by employing qRT-PCRe.A total of 29 AMP genes were identified,including 12 cecropins,11 defensins,4 attacins,and 2 diptericins.The genomic and protein structures indicate that B.dorsalis AMP molecules and structures exhibit genetic diversity mechanisms,including gene duplication.The expression profiles of AMPs at different developmental stages revealed that most AMPs are highly expressed during the pupal and adult stages.The expression characteristics of different types of AMPs varied significantly in different body parts,and apart from the hemolymph,individual genes were also expressed in the head and integument.These findings provide a database and information for further research on the functions of B.dorsalis AMPs.