广东湛江西番莲的主要病毒的分子检测与分析

Molecular detection and analysis of main viruses infecting Passiflora edulis planted in Zhanjiang,Guangdong Province

采集西番莲(Passiflora edulis)品种‘钦蜜9号’的脱毒种苗和田间疑似患病植株的叶片为材料,提取其核酸,根据马铃薯Y病毒属(Potyvirus)的西番莲木质化病毒、西番莲斑驳病毒、东亚西番莲病毒、夜来香花叶病毒,菜豆金色花叶病毒属(Begomovirus)的西番莲扭叶病毒、大戟曲叶病毒、广东番木瓜曲叶病毒和黄瓜花叶病毒属(Cucumovirus)的黄瓜花叶病毒的保守序列设计8对检测引物,PCR检测样品内的病毒种类。从西番莲脱毒种苗样品中没有扩增得到任何病毒的片段;田间患病的西番莲样品中一共扩增得到5条可能的病毒片段,经测序后确认为马铃薯Y病毒属(Potyvirus)东亚西番莲病毒EAPV,夜来香花叶病毒TeMV和菜豆金色花叶病毒属(Begomovirus)的大戟曲叶病毒EuLCV,广东番木瓜曲叶病毒PaLCuGDV。序列比对和系统进化树分析结果表明PCR产物与韩国和中国的台湾省、福建省等地的病毒毒株有较高的同源性。确认在广东湛江种植的西番莲品种‘钦蜜9号’植株携带病毒EAPV、TeMV、EuLCV、PaLCuGDV。本研究也是广东地区首次在西番莲中检测到病毒EAPV、PaLCuGV和EuLCV。

Leaves of virus-free seedlings of Passiflora edulis‘Qinmi 9’and suspected diseased P.edulis plants were collected for extraction of nucleic acids.P.edulis virus detection primers were designed according to the conserved regions of P.edulis woodiness virus,P.edulis mottle virus,East Asian Passiflora virus,Telosma mosaic virus,P.edulis leaf distortion virus,Euphorbia leaf curl virus,Papaya leaf curl Guangdong virus and Cucumber mosaic virus.,and the virus species in different samples were identified by using PCR method.No suspected virus fragments were amplified from the virus-free seedlings of P.edulis.A total of five possible virus fragments were amplified from suspected infected P.edulis seedling samples in the field.They were identified by sequencing as East Asian P.edulis virus,Telosma mosaic virus and Euphorbia leaf curl virus,Papaya leaf curl Guangdong virus.Sequence alignment and phylogenetic tree analysis showed that the PCR products had a high homology with the virus strains from Taiwan Province of China,the Republic of Korea,Fujian Province of China and other regions.The‘Qinmi 9’plant planted locally in Zhanjiang was confirmed to carry the virus EAPV,TeMV,EuLCV,PaLCuGDV.This is also the first time that viruses EAPV,PaLCuGV and EuLCV were detected in P.edulis in Guangdong,China.