利用间歇浸没式生物反应器进行橡胶组培快繁研究

Rapid propagation of rubber tree by using intermittentimmersion Bioreactor

为了寻找橡胶树组培育苗的高效途径,以‘RRIM 600’橡胶果内珠被初萌体胚为材料,通过筛选激素配比、优化接种密度和间歇频率等培养条件,探索RITA?间歇浸没式生物反应器培养橡胶果内珠被体细胞胚的适宜条件。结果表明,‘RRIM 600’果内珠被初萌体胚间歇浸没式培养的最佳条件为:MS+1.0 mg·L^(-1)6-BA+0.1 mg·L^(-1)NAA+50 g·L^(-1)蔗糖,营养液间歇浸没频率为每6 h浸没处理1 min,接种密度为每个容器15个体胚。与固体培养相比,该条件下体胚再生植株的株高、茎粗、根长、生根数和单株鲜质量分别显著增加了30%、91.7%、34.8%、53.3%和97.2%;再生植株的SOD、CAT和POD协调作用能力更强。RITA?间歇浸没培养方式是提高橡胶树初萌体胚形成再生植株效率的有效途径,可缩短橡胶树体胚再生植株形成周期及提高体胚苗生长质量。

The integument somatic embryos of rubber tree of clone RRIM 600 were cultured in an intermittent immersion bioreactor RITA®,and the suitable conditions for somatic embryo culture of rubber tree were established.The types of hormones were screened and the culture conditions were optimized.The experimental results showed that the best condition for intermittent immersion culture of RRIM 600 rubber integument primordium-derived somatic embryos were:MS+1.0 mg·L^(-1)6-BA+0.1 mg·L^(-1) NAA+50 g·L^(-1) sucrose as the medium,with the intermittent immersion frequency of nutrient solution being 1 min/6 h,and the inoculation density being 15 individual embryos/container.Compared with solid culture,the intermittent immersion culture under this condition increased significantly the plant height,stem diameter,root length,root number and fresh weight per plant of somatic embryo regeneration plants by 30%,91.7%,34.8%,53.3%and 97.2%,respectively.The coordination ability of SOD,CAT and POD in regenerated plants was higher under this condition,which was an effective way to improve the efficiency of regeneration plant formation from primary somatic embryos of rubber tree.All these results showed that the use of intermittent immersion culture method RITA®could shorten the formation cycle of somatic embryo regeneration plants and improve the growth quality of somatic embryo plants of rubber tree.