枯草芽孢杆菌对耐药维氏气单胞菌小鼠肠道感染的预防效果评价

Evaluation of the therapeutic effect of Bacillus subtilis on the intestinal tract of mice infected by Aeromonas veronii

作为一种人畜共患多药耐药菌,维氏气单胞菌(Aeromonas veronii,A.veronii)感染的治疗和预防主要是使用抗生素。然而,抗生素的大量使用极易造成新耐药突变株的出现,开发无抗生防制剂抑制致病菌感染成为当前发展趋势。枯草芽孢杆菌(Bacillus subtilis,B.subtilis)作为常见的益生菌,对多种致病菌具有抑制作用。本文选择B.subtilis作为研究对象,研究其对A.veronii的抑制作用,评估其对肠道感染的治疗效果。滤纸片实验结果表明B.subtilis对A.veronii C4具有明显的抑制作用;TEM结果表明,B.subtilis会造成A.veronii C4细胞胀裂、破损。此外,B.subtilis可以降低A.veronii C4的细胞毒性。在肠道屏障遭到破坏的小鼠模型中,B.subtilis不仅可以修复受损的肠道屏障,还能有效抵御A.veronii C4对小鼠器官、肠道的损伤。B.subtilis可作为治疗A.veronii感染的生防制剂,对于遏制A.veronii耐药性发展具有重要意义。

Infection of Aeromonas veronii(A.veronii),a kind of zoonotic multidrug-resistant bacteria,is mainly treated or prevented with antibiotics.However,the extensive use of antibiotics can lead to the emergence of new drug-resistant mutant strains.Bacillus subtilis(B.subtilis),as a common probiotic,which can inhibit various species of pathogenic bacteria.The antibacterial ability of B.subtilis against A.veronii was detected and its therapeutic effect on A.veronii intestinal infection evaluated.Filter paper method showed that B.subtilis possessed an obvious inhibitory effect on A.veronii C4,and TEM showed that it caused the dilatation and damage of A.veronii C4 cells.In addition,B.subtilis reduced the intracellular cytotoxicity of A.veronii C4.In a mouse model with damaged intestinal barrier,B.subtilis showed higher efficiency than the clinical antibiotic on A.veronii C4 infections,contributing to their natural ability of intestinal barrier repair and the antibacterial ability against A.veronii C4.B.subtilis can thus be used as a biocontrol agent for the treatment of A.veronii infection,which is of great significance for curbing the development of drug-resistance.