布鲁氏菌A19和A19-△VirB12疫苗接种犊牛的免疫抗体变化比较

Changes in Immune Antibodies in Calves Vaccinated with Brucella A19 Vaccine and A19-△VirB12 Vaccine

为了解犊牛采用WOAH推荐的A19疫苗的免疫方式接种不同剂量的A19疫苗和A19-△VirB12疫苗的抗体变化,应用不同剂量的布鲁氏菌A19疫苗和A19-△VirB12疫苗采用WOAH推荐的黏膜免疫和皮下接种两种免疫方式接种3~6月龄母犊牛,采用试管凝集试验(SAT)检测采集于犊牛免疫各时段的血清中抗体变化。结果显示,试验一中犊牛皮下接种常规剂量A19疫苗和A19-△VirB12疫苗在免疫2周均会产生较高水平的抗体。在免疫24周两组仍有5%~15%的血清抗体效价维持在1:200以上。试验二中采用1/10常规剂量的A19疫苗和A19-△VirB12疫苗通过黏膜和皮下免疫犊牛,各组在免疫15周均出现较高水平的抗体效价,在免疫30周,两个皮下免疫组仍有11.76%的血清抗体效价维持在1:200以上。试验结果表明,犊牛皮下接种常规剂量A19-△VirB12疫苗与接种常规剂量A19疫苗产生的抗体效价水平相当。犊牛黏膜免疫和皮下免疫1/10剂量的A19-△VirB12疫苗与同一方法接种同一剂量A19疫苗产生的抗体效价水平相当。

In order to understand the antibody changes of calves inoculated with different doses of A19 vaccine and A19-△VirB12 vaccine according to WOAH recommended A19 vaccine.Different doses of brucella A19 vaccine and A19-△VirB12 vaccine were administered to 3~6 month old female calves by mucosal immunization and subcutaneous immunization as recommended by WOAH,and the changes of serum antibodies collected at different immunization periods were detected by test-tube agglutination test(SAT).Results showed that,in trial 1,subcutaneous administration of conventional doses of A19 vaccine and A19-△VirB12 vaccine produced higher levels of antibodies at 2 weeks of immunization.At 24 weeks of immunization,5%~15%of serum antibody titers in both groups remained above 1:200.In trial 2,1/10 of conventional doses of A19 vaccine and A19-△VirB12 vaccine were used to immunize calves through mucosa and subcutaneously.High antibody titers were found in all groups at the 15th week of immunization,and 11.76%of serum antibody titers in the two subcutaneously immunized groups remained above 1:200 at the 30th week of immunization.Subcutaneous administration of the conventional dose of A19-△VirB12 vaccine in calves produced antibody titer levels comparable to that of the conventional dose of A19 vaccine.Calf mucosal immunization and subcutaneous immunization 1/10 doses of A19-△VirB12 vaccine produced antibody titer levels comparable to the same dose of A19 vaccine administered by the same method.