新疆传统发酵乳制品中肠球菌的分离鉴定

Isolation and identification of Enterococcus from traditional fermented dairy products in Xinjiang

该研究采集了新疆地区传统发酵乳制品,对其中的乳酸菌进行分离培养,将分离筛选乳酸菌进行镜检、16S rRNA基因测序和API 50 CHL糖发酵实验。结果表明,共筛选出7株肠球菌,其中菌株916021、23150、AQH21-3和130被鉴定为粪肠球菌(Enterococcus faecalis),菌株622331、G22381和YL-CX-2-1被鉴定为屎肠球菌(Enterococcus faecium)。API 50 CHL糖发酵实验结果发现,菌株AQH21-3不可利用丙三醇、山梨醇、苦杏仁背、D-塔格糖,可利用L-阿拉伯糖;菌株916021、130可以利用D-蔗糖、D-松三糖,菌株916021还可利用L-鼠李糖。菌株G22381、YL-CX-2-1无法利用丙三醇、山梨醇、苦杏仁苷、葡萄糖酸钾;与其他两株屎肠球菌相比,菌株G22381可利用L-阿拉伯糖,但无法利用D-塔格糖。结果显示肠球菌在碳水化合物利用上存在多样性,为后续肠球菌资源的进一步开发研究提供数据参考。

In this study,the traditional fermented dairy products in Xinjiang were collected and the lactic acid bacteria were isolated and cultured.The isolated lactic acid bacteria were subjected to microscopic examination,16S rRNA gene sequencing and API 50 CHL sugar fermentation experiment.The results showed that a total of 7 strains of Enterococcus were selected,among which strains 916021,23150,AQH21-3 and 130 were identified as Enterococcus faecalis.Strains 622331,G22381 and YL-CX-2-1 were identified as Enterococcus faecium.The results of API 50 CHL sugar fermentation results showed that strain AQH21-3 could not use glycerol,sorbitol,amygdon and D-tagose,but could use L-arabinose.Strains 916021 and 130 could use D-sucrose and D-pine triose,and strains 916021 could also use L-rhamnose.Strains G22381 and YL-CX-2-1 could not utilize glycerol,sorbitol,amygdalin and potassium gluconate.Compared with the other two E.faecium strains,strain G22381 could utilize L-arabinose but could not utilize D-tagose.The results showed that Enterococcus had diversity in carbohydrate utilization,which provided data reference for further development of Enterococcus resources.