枸杞水溶性抗氧化物质提取工艺优化及其活性成分分析

Optimization of extraction process and analysis of active components of water-soluble antioxidant substances from Goji

该研究采用水提法对枸杞中水溶性物质进行提取,在单因素试验的基础上,以清除1,1二苯基-2-三硝基苯肼(DPPH)自由基的半抑制浓度(IC50)值为响应值,采用响应面试验优化提取工艺,并通过超高效液相色谱串联四级杆飞行时间质谱(UPLC-QTOF-MS)对枸杞水溶性抗氧化成分进行分析。结果表明,最佳提取工艺为料液比1∶180(g∶mL)、提取温度80℃、提取时间2 h。在此优化条件下,提取液清除DPPH自由基的IC50值为1.34 g/L。通过UPLC-QTOF-MS分析表明,枸杞水提液中检出6个清除DPPH自由基的化合物,分别为枸杞酸、甜菜碱、山柰酚-3-O-槐二糖-7-O-葡萄糖苷、脯氨酸、L-精氨酸和芦丁。

The water-soluble substances in Goji was extracted by aqueous extraction method.Using half maximal inhibitory concentration(IC50)value of scavenging 1,1-diphenyl-2-picrylhydrazyl(DPPH)radicals as response value,the extraction process was optimized by response surface tests on the basis of single factor tests.The water-soluble antioxidant components in Goji were analyzed by ultra performance liquid chromatography coupled with quadrupole-time of flight-mass spectrometry(UPLC-QTOF-MS).The results showed that the optimal extraction conditions were as follows:solid-liquid ratio 1∶180(g∶ml),extraction temperature 80℃and time 2 h.The IC50 value of scavenging DPPH radicals of the extracting solution was 1.34 g/L under these optimized conditions.The analysis by UPLC-QTOF-MS indicated that a total of 6 substances capable of scavenging DPPH radicals were detected in the aqueous extracting solution of Goji,which were beriberic acid,betaine,kaempferol-3-O-sophorodisaccharide-7-O-glucoside,proline,L-arginine and rutin,respectively.