仔猪溃疡性结肠炎模型的建立分析

Establishment and Analysis of Piglet Ulcerative Colitis Model

该研究旨在探索并确立一种可靠的乙酸诱导仔猪溃疡性结肠炎模型。试验选取了18头健康的杜洛克仔猪作为研究对象,将其随机分配至对照组、TNBS组以及乙酸组,每组仔猪麻醉后直肠灌注不同试剂,包括生理盐水、不同浓度的三硝基苯磺酸50%乙醇溶液以及不同浓度的乙酸溶液,旨在诱发不同程度的结肠炎病变。观察期内详细记录仔猪的临床表现与粪便特征,并在灌注3~7 d后进行宰杀,收集结肠组织进行结肠黏膜损伤评估。研究结果指出,直肠灌注10%乙酸能够成功建立仔猪溃疡性结肠炎模型。该研究以期为深入理解溃疡性结肠炎的发病机制、药物筛选及治疗策略提供一个稳定且有效的动物模型平台。

The aim of this study was to explore and establish a reliable model of ulcerative colitis induced by acetic acid in piglets.Eighteen healthy Duroc piglets were selected as the study subjects and rando mLy assigned to the control group,TNBS group and acetic acid group.Different reagents were injected into the rectum of each group after anesthesia,including normal saline,TNBS 50%ethanol solution of different concentrations and acetic acid solution of different concentrations to induce colitis lesions of different degrees.The clinical manifestations and fecal characteristics of piglets were recorded in detail during the observation period,and the piglets were slaughtered after 3 to 7 days of perfusion,and the colon tissue was collected for colon mucosal injury assessment.The results indicate that rectal infusion of 10%acetic acid can successfully establish the model of ulcerative colitis of piglets.This study aims to provide a stable and effective animal model platform for further understanding the pathogenesis,drug screening and treatment strategies of ulcerative colitis.