1例牛混合感染多杀性巴氏杆菌和化脓隐秘杆菌的诊断与治疗

A Case of Bovine Co-infection with Pasteurella Multocida and Arcanobacterium Pyogenes Diagnosis and Treatment

2023年12月8日广西某奶牛场出现有5~6头泌乳母牛有流浓鼻涕、呼吸急促、体温升高现象,奶量急速下降,且疾病具有传染性,牛群出现死亡现象,对病死牛解剖观察发现病死牛呈现全身败血性病变,初步还以为细菌性感染,为进一步确诊病原,无菌采集牛肺、肝脏、脾脏、肠道、血液、粪便、鼻液等样品病原PCR检测和细菌分离,对分离菌进行16S rRNA扩增鉴定以及混菌药敏试验。结果显示样品病原PCR检测中多杀性巴氏杆菌和化脓隐秘杆菌呈阳性,分离得到2株菌,经16S rRNA扩增鉴定这2株菌分别为多杀性巴氏杆菌和化脓隐秘杆菌,混菌药敏试验显示这2株菌对大观霉素、恩诺沙星、环丙沙星、头孢噻呋较为敏感,对卡那霉素、阿莫西林、氧氟沙星、多粘菌素中度敏感。根据临床和实验室诊断确认此次疫情是由多杀性巴氏杆菌与化脓隐秘杆菌混合感染,为及时准确采取针对性治疗措施提供了参考。

On December 8,2023,a dairy farm have 5~6 lactating cows flow thick nose,shortness of breath temperature,milk volume drops rapidly,and disease is infectious,cattle died,the dead cattle anatomical observation found dead cattle present systemic hemorrhagic disease,preliminary thought that bacterial infection,for further diagnosis pathogen,sterile collection cattle lung,liver,spleen,intestinal,blood,feces,nasal fluid samples pathogen PCR detection and bacterial isolation,the bacteria 16S rRNA amplification identification and mixed susceptibility test.The results show that the sample pathogen PCR detection multocidal and pyogenes positive,isolated two strains,after 16S rRNA amplification identified the two strains are multocidal and pyogenes,mixed susceptibility test showed that the two strains of spectinomycin,enrofloxacin,ciprofloxacin,ceftiofur,sensitive to kanamycin,amoxicillin,ofloxacin,polymyxin moderately sensitive.According to clinical and laboratory diagnosis,the outbreak was confirmed as a mixed infection of P.multiple bacteria and M.pyogenes,which provides a reference for timely and accurate targeted treatment measures.