浅谈CRISPR系统及其应用现状

Discussion on CRISPR System and Its Application Status

随着技术的不断发展,一项新兴基因编辑技术——CRISPR/Cas9,逐渐进入公众视野。CRISPR/Cas9系统最初被发现于细菌中,后被研究者作为第三代基因编辑技术。相较于前两代技术,CRISPR/Cas9在基因敲除和基因插入方面展现出更高效的效率和精准度。此项技术持续优化,已能运用于真核生物的疾病治疗等方面,并展现出巨大的发展潜能。研究者指出,限制此项技术应用的只有想象力。基于此,本文将从该技术的发现背景、优化历程、主要作用以及在小鼠和线虫中的应用进行阐述,期待为相关工作人员提供一些支持与帮助。

With the continuous development of technology,an emerging gene editing technology,CRISPR/Cas9,has gradually entered the public eye.This system was first discovered in bacteria and later used by researchers as a third-generation gene editing technology.Compared with the previous two generations of technology,CRISPR/Cas9 has demonstrated greater efficiency and precision in gene knockout and gene insertion.This technology continues to be optimized and can be applied to the treatment of eukaryotic diseases,showing great development potential.Researchers have pointed out that the only thing that limits the use of this technology is imagination.In this article,we will elaborate on the background of the discovery of this technology,its optimization process,its main role,and its application in mice and nematodes,looking forward to providing some support and assistance to the staff concerned.