川芎内生真菌隔孢伏革菌Peniophora incarnata的次生代谢产物

Secondary metabolites of endophytic fungus Peniophora incarnata from Ligusticum chuanxiong Hort.

对川芎(Ligusticum chuanxiong Hort.)内生真菌隔孢伏革菌Peniophora incarnata的次生代谢产物进行研究.用大米蛋白胨固体培养基发酵培养,乙酸乙酯浸泡提取后,通过凝胶柱层析、硅胶柱层析及制备高效液相等多种分离手段对次生代谢产物进行分离纯化研究,共分离鉴定了20个化合物,分别为麦角甾醇(1)、麦角甾-4,6,8(14),22-四烯-3-酮(2)、β-谷甾醇(3)、麦角甾7,22-二烯-3,6-二酮(4)、5-hydro-xymethyl-2-furancarboxaldehyde(5)、5-乙酰氧甲基糠醛(6)、(1S,2R)-(-)-1-phenylpropane-1,2-diol(7)、(±)-3-phenyllactic acid(8)、ethyl 2-hydroxy-3-phenylpropanoate(9)、(R)-3-苯基乳酸甲酯(10)、3-phenylpropane-1,2-diol(11)、1-phenyl-1,3-diacetate(12)、对羟基苯乙酸(13)、4′-羟基苯乙酸-2,3-丁二醇-2-酯(14)、香草酸(15)、对羟基苯甲酸乙酯(16)、2-(4-羟苯基)乙酸乙酯(17)、阿魏酸(18)、2-(4-hydroxyphenyl)-ethanol(19)、对羟基苯甲酸乙酯(20),除化合物16和18,其余18个化合物均为首次从隔孢伏革菌的发酵产物中分离得到,且此菌中含有与川芎相同的酚酸类化合物.测试所有化合物的α-葡萄糖苷酶抑制活性,评价其降糖活性,本研究表明化合物2具有较好的降糖活性,其半数抑制浓度(IC50)为0.860±0.046 mmol/mL,远低于阳性对照阿卡波糖7.057±0.005 mmol/mL.(图2参48)

This study was conducted to evaluate the secondary metabolites of Peniophora incarnata,an endophytic fungus of Ligusticum chuanxiong Hort.Peniophora incarnata was grown in solid-substrate fermentation culture.Compounds were isolated using gel column chromatography,silica gel column chromatography,and simi-prepared high-performance liquid chromatography.The structures of the compounds were identified using spectroscopic methods.Twenty compounds were isolated from the secondary metabolites and identified as ergosterol(1),ergostane 4,6,8(14),22-tetraene-3-one(2),β-sitosterol(3),ergosta-7,22-diene-3,6-dione(4),5-hydroxymethyl-2-furancarbox-aldehyde(5),5-(acetoxymethyl)furfural(6),(1S,2R)-(-)-1-phenylpropane-1,2-diol(7),(±)-3-phenyllactic acid(8),ethyl 2-hydroxy-3-phenylpropanoate(9),(R)-3-phenyl lactic acid methyl ester(10),3-phenylpropane-1,2-diol(11),1-phenyl-1,3-diacetat(12),p-hydroxyphenylacetic acid(13),4′-hydroxy-phenylacetic acid,2,3-butanediol,2-ester(14),vanillic acid(15),ethyl p-hydroxybenzoate(16),2-(4-hydroxyphenyl)ethyl acetate(17),ferulic acid(18),2-(4-hydroxyphenyl)-ethanol(19),and 2-p-acetoxyphenylethanol(20).Except for compounds 16 and 18,the other 18 compounds were isolated from the secondary metabolites of P.incarnata for the first time,and the endophytic fungus contained the same phenolic acids as L.chuanxiong Hort.All compounds were tested for theirɑ-glucosidase inhibitory activity to determine their hypoglycemic activity.Compound 2 exhibited good hypoglycemic activity.The half-maximal inhibitory concentration was 0.860±0.046 mmol/mL,which was much lower than that of acarbose(7.057±0.005 mmol/mL).