FecB基因突变对绵羊超排处理效果的影响

Effects of FecB Gene Mutation on Superovulation Treatment in Sheep

为了探究FecB基因突变对绵羊同期发情-超数排卵效果的影响,基于同期发情、超数排卵等技术处理,对3种FecB基因型小尾寒羊进行了处理前后的发情时间和卵泡数量统计研究,分析其外周血清激素和氧化应激指标的差异。结果表明,相比于同期非超排试验处理,超排组绵羊群体的发情时间更为集中;且携带FecB基因突变的BB和+B型绵羊群体,在超排激素作用下所形成的排卵前卵泡数显著高于++野生型(P<0.05)。在发情后6 h超排处理的BB型绵羊外周血清FSH浓度水平显著高于其他各组(P<0.05),预示着绵羊发情早期的FSH浓度可能决定着随后的卵泡形成数量。超排处理后的3种FecB基因型绵羊群体之间的外周血清氧化应激水平差异不显著(P>0.05);而非超排处理的BB型较高的血清MDA水平,反应了正常生理水平下BB型绵羊较高的卵泡形成数量可能引起了机体的相当程度的高氧化应激状态。

In order to explore the effect of FecB gene mutation on the effect of simultaneous estrus-superovulation in sheep,statistical studies on estrus time,follicle number,hormones concentration and oxidative stress indexes in peripheral serum for three FecB genotypes of Small Tail Han sheep before and after the superovulation treatment were conducted.The results showed that the estrus time of the sheep group stimulated by superovulation hormone was more concentrated than the non-superovulation treatment group;and the number of preovulatory large follicles formed in BB and+B sheep which carrying FecB gene mutation was significantly higher than that of++wild type under stimulation by superovulation hormone(P<0.05).At 6 hours after estrus,the peripheral serum FSH concentration of BB sheep treated with superovulation was significantly higher than that of other groups(P<0.05),indicating that the FSH concentration in the early estrus may determine the number of subsequent follicle formation.There was no significant difference in peripheral serum oxidative stress levels among the three FecB genotype sheep populations after superovulation treatment(P>0.05);while the higher level of serum MDA for the non-superovulation in BB genotype sheep reflects the higher number of follicles formed may cause a considerable degree of high oxidative stress in the sheep under normal physiological levels.