木质素接枝聚乳酸微球制备及骨细胞培养研究

Preparation of lignin⁃grafted⁃poly(L−lactide) microspheres and bone cell culture

木质素基可降解材料近年来在生物医用领域受到越来越多的关注,但目前其在细胞培养微载体上的应用还有待进一步开发。研究利用烷基化的碱木质素引发左旋丙交酯开环聚合,首次采用不同分子量的木质素接枝聚乳酸(LGPL-L, LGPL-H)制备微球。通过仿生矿化评估不同微球材料诱导矿化的能力,反映其骨生物活性。将不同微球与MG-63(人骨肉瘤细胞)成骨细胞共培养后,不同微球材料组中的细胞活力如下:LGPL-L>LGPL-H>PLLA(左旋聚乳酸)。实验结果表明:相比聚乳酸微球,木质素基聚乳酸微球诱导矿化的能力较强,与人成骨细胞共培养后的黏附和增殖效果更好,并揭示了其作为细胞培养载体的应用潜力。

Lignin‑based degradable materials have received increasing attention in the biomedical field in recent years,but there have been few reports of their application in cell culture microcarriers.In this work,alkylated alkali lignin was used as an initiator for the ring‑opening polymerization of L-lactide.Lignin‑grafted‑poly(L-lactide)(LGPL-H,LGPL-L)samples with different molecular weights were used to prepare microspheres for the first time,and then co-cultured with MG-63 osteoblasts.The ability of the microspheres to induce mineralization was evaluated through biomimetic mineralization,which reflects their bone bioactivity.The cell viabilities of different microspheres decreased in the order LGPL-L>LGPL-H>PLLA.These results show that lignin‑based polylactic acid microspheres can promote the adhesion and proliferation of human osteoblasts,confirming their potential for application in cell culture microcarriers.