黄芩苷调节SDF-1/CXCR4信号通路对高糖诱导的心肌细胞凋亡的影响

Effect of Baicalin on High-Glucose Induced Apoptosis of Myocardial Cells by Regulating the SDF-1/CXCR4 Signaling Pathway

目的:探讨黄芩苷调节基质细胞衍生因子1(SDF-1)/趋化因子受体4(CXCR4)信号通路对高糖(HG)诱导的心肌细胞凋亡的影响。方法:以H9C2心肌细胞为研究对象,分为对照组、HG组、黄芩苷低浓度组(L-Ba组)、黄芩苷中浓度组(M-Ba组)、黄芩苷高浓度组(H-Ba组)、黄芩苷+CXCR4抑制剂组(Ba+AMD3100组)。CCK-8法检测各组细胞活力;流式细胞仪检测各组细胞凋亡率;qRT-PCR检测各组细胞中Bax、Bcl-2的mRNA表达;微板法检测各组细胞中SOD活力和MDA含量;免疫印迹法(WB)检测各组细胞中SDF-1、CXCR4蛋白表达水平。结果:与对照组比较,HG组存活率、Bcl-2 mRNA相对表达量、SOD活性及SDF-1和CXCR4蛋白表达水平降低,心肌细胞凋亡率、Bax mRNA相对表达量及MDA含量升高,差异均有统计学意义(P<0.05);与HG组比较,L-Ba组、M-Ba组、H-Ba组存活率、Bcl-2 mRNA相对表达量、SOD活性及SDF-1和CXCR4蛋白表达水平升高,心肌细胞凋亡率、Bax mRNA相对表达量及MDA含量降低,且随着黄芩苷浓度增加呈逐渐上升或下降趋势,差异均有统计学意义(P<0.05);Ba+AMD3100组心肌细胞存活率、Bcl-2 mRNA相对表达量、SOD活性、SDF-1、CXCR4蛋白表达水平低于L-Ba组、M-Ba组、H-Ba组,心肌细胞凋亡率、Bax mRNA相对表达量、MDA含量高于L-Ba组、M-Ba组、H-Ba组,差异均有统计学意义(P<0.05)。结论:黄芩苷可通过激活SDF-1/CXCR4信号通路缓解HG诱导的心肌细胞凋亡。

Objective:To investigate the effect of baicalin on high glucose(HG)induced apoptosis of myocardial cells by regulating the stromal cell derived factor 1(SDF-1)/chemokine receptor 4(CXCR4)signaling pathway.Methods:H9C2 myocardial cells were used as the research object and grouped into control group,HG group,low concentration baicalin group(L-Ba group),medium concentration baicalin group(M-Ba group),high concentration baicalin group(H-Ba group),and baicalin+CXCR4 inhibitor group(Ba+AMD3100 group).CCK-8 method was applied to detect cell viability in each group;flow cytometry was applied to detect the apoptosis rate of cells in each group;qRT-PCR was applied to detect the mRNA expression of Bax and Bcl-2 of cells in each group;microplate method was applied to detect SOD activity and content of MDA of cells in each group;Western blot(WB)was applied to detect the expression levels of SDF-1 and CXCR4 proteins of cells in each group.Results:Compared with the control group,the survival rate,relative expression of Bcl-2 mRNA,SOD activity,and expression levels of SDF-1 and CXCR4 proteins in the HG group decreased,the apoptosis rate of myocardial cells,relative expression of Bax mRNA,and content of MDA increased(P<0.05);compared with the HG group,the survival rate,relative expression of Bcl-2 mRNA,SOD activity,and expression levels of SDF-1 and CXCR4 proteins in the L-Ba group,M-Ba group,H-Ba group increased,the apoptosis rate of myocardial cells,relative expression of Bax mRNA,and content of MDA decreased,and showed gradual upward or downward trend as the concentration of baicalin increased(P<0.05);the survival rate of myocardial cells,relative expression of Bcl-2 mRNA,SOD activity,and protein expression levels of SDF-1 and CXCR4 in the Ba+AMD3100 group were lower than those in the L-Ba,M-Ba,and H-Ba groups,the apoptosis rate of myocardial cells,relative expression of Bax mRNA,and content of MDA were higher in the L-Ba group,M-Ba group,and H-Ba group(P<0.05).Conclusion:Baicalin can alleviate HG-induced apoptosis of myocardial cells by activating the SDF-1/CXCR4 signaling pathway.