口腔鳞状细胞癌患者铜死亡相关基因预后模型的构建和验证

Construction and validation of cuproptosis-related genes prognostic model for oral squamous cell carcinoma

目的构建并验证口腔鳞状细胞癌(OSCC)患者铜死亡相关基因预后风险模型。方法在肿瘤基因组图谱(TCGA)数据库中下载OSCC患者临床及转录组数据,筛选出OSCC患者铜死亡相关基因,使用共表达分析、Wilcoxon秩和检验、Cox分析和AIC逐步算法构建铜死亡相关基因风险模型,并评估其准确性。结果共筛选出340例OSCC患者作为训练组和内部验证组,24例患者作为外部验证组,成功建立了OSCC患者铜死亡相关基因风险模型。该模型由细胞周期蛋白依赖性激酶调节亚基2(CKS3)、三角形四肽重复域21A(TTC21A)、锌指含MYND型10(ZMYND10)3个铜死亡相关基因构成,受试者工作特征(ROC)曲线的曲线下面积(AUC)为0.710,具有理想的预测性能。其中风险评分高的OSCC患者预后相对较差,并且上述模型评分是OSCC患者预后的一个独立影响因素(HR=1.472,95%CI:1.141~1.899,P=0.003)。结论本研究构建的模型可为OSCC患者的诊疗和预后评估提供可靠依据。

Objective To construct and verify the prognostic risk model for cuproptosis-related genes in oral squamous cell carcinoma(OSCC).Methods The clinical and transcriptomic data of OSCC patients were downloaded from the Cancer Genome Atlas(TCGA)database.The cuproptosis-related genes in the patients with OSCC were screened out.The cuproptosis-related genes risk model was constructed by the coexpression analysis,Wilcoxon rank sum test,Cox analysis and AIC step-by-step algorithm.Then its accuracy was evaluated.Results A total of 340 cases of OSCC were screened out and served as the training group and internal verification group.A total of 24 cases served as the external verification group.The cuproptosis-related genes risk model in the patients with OSCC was successfully constructed.This model was composed of cyclin dependent kinases regulatory subunit 2(CKS2),tetratricopeptide repeat domain 21A(TTC21A)and zinc finger,MYND-type containing 10(ZMYND10)copper death-related genes.The area under the receiver operating characteristic(ROC)curve(AUC)was 0.710,possessing the ideal predictive performance.Among them,the prognosis in the OSCC patients with high risk score was relatively poor.In addition,the above model score was an independent influencing factor for the prognosis in OSCC patients(HR=1.472,95%CI:1.141-1.899,P=0.003).Conclusion The constructed model could provide the reliable basis for the diagnosis,treatment and prognosis evaluation in the patients with OSCC.