Elucidation of cellular signaling mechanism involved in Vibrio cholerae chitin-binding protein GbpA mediated IL-8 secretion in the intestinal cells

Background:Vibrio cholerae N-acetylglucosamine-binding protein(GbpA)is a four-domain,secretory colonization factor which is essential for chitin utilization in the environment,as well as in adherence to intestinal cells.GbpA is also involved in inducing intestinal inflammation by enhancing mucin and interleukin-8 secretion.The underlying cell signaling mechanism involved in the induction of the pro-inflammatory response and IL-8 secretion has yet to be deciphered in detail.Methods:Herein,the process through which GbpA triggers the induction of IL-8 in intestinal cells was investigated by examining the role of GbpA in intestinal cell line HT 29.Results:GbpA,specifically through the fourth domain,forms a binding connection with Toll-like receptor 2(TLR2)and additionally,recruits TLR1 along with CD14 within a lipid raft micro-domain to initiate the signal-ing pathway.Notably,disruption of this micro-domain complex resulted in a reduction in IL-8 secretion.The lipid raft association served as the catalyst that invoked a downstream cellular inflammatory signaling path-way.This cascade involved the activation of various MAP kinases and NF𝜅B and assembly of the AP-1 complex.This coordinated activation of signaling molecules eventually leads to enhanced IL-8 transcription via increased promoter activity.These findings suggested that GbpA is a crucial protein in V.cholerae,capable of inciting a pro-inflammatory response during infection by orchestrating the formation of the GbpA-TLR1/2-CD14 lipid raft complex.Activation of AP-1 and NF𝜅B in the nucleus eventually enhanced IL-8 transcription through increased promoter activity.Conclusions:Collectively,these findings indicated that GbpA plays a pivotal role within V.cholerae by triggering a pro-inflammatory response during infection.This response is instrumented by the formation of the GbpA-TLR1/2-CD14 lipid raft complex.