月季RhMYB25基因的克隆和非生物胁迫下的检测分析

Cloning and analysis of RhMYB25 gene in rose under abiotic stress

该文研究RhMYB25转录因子在月季(Rosa hybrida)非生物胁迫下的表达模式,为进一步揭示RhMYB25基因的功能奠定基础。利用月季全基因组数据,采用RT-PCR从月季叶片中分离得到1个MYB转录因子,命名为RhMYB25,并对其进行生物信息学分析;采用烟草瞬时表达技术,明确RhMYB25转录因子的亚细胞定位;利用实时荧光定量PCR技术分析RhMYB25在不同组织及其不同非生物胁迫下的表达模式。RhMYB25基因编码区长度为1032bp,编码343个氨基酸,包含1个R2R3-MYB结构域,其属于典型的MYB转录因子。亚细胞定位显示RhMYB25定位于细胞核。实时荧光定量PCR结果表明,RhMYB25基因在月季叶片中的表达最高;高温与脱水胁迫可以显著诱导RhMYB25基因的表达,但外源盐处理不能诱导RhMYB25基因的表达。RhMYB25基因可能在月季响应高温与脱水胁迫过程中发挥调控作用。

To study the expression patterns of RhMYB25 transcription factors under abiotic stress in roses(Rosa hybrida),and to lay the foundation for further revealing the function of the RhMYB25 gene.Using whole genome data of roses,a MYB transcription factor named RhMYB25 was isolated from rose leaves using RT-PCR(reverse transcription-polymerase chain reaction)and subjected to bioinformatics analysis;using tobacco transient expression technology to clarify the subcellular localization of RhMYB25;using real-time fluorescence quantitative(polymerase chain reaction)technology to analyze the expression patterns of RhMYB25 in different tissues and under different abiotic stresses.The coding region length of the RhMYB25 gene is 1032 bp,encoding 343 amino acids and containing one R2R3-MYB domain,which belongs to a typical MYB transcription factor.Subcellular localization shows that RhMYB25 is located in the nucleus.The real-time fluorescence quantitative PCR results showed that RhMYB25 had the highest expression in rose leaves;high temperature and dehydration stress can significantly induce the expression of RhMYB25,but exogenous salt treatment cannot induce the expression of RhMYB25.RhMYB25 play a regulatory role in the response of roses to high temperature and drought stress.