当归补血汤补血作用的谱效关系研究

Spectrum-Effect Relationship of Danggui Buxue Decoction(当归补血汤)for Hematopoietic Effect

目的:研究当归补血汤不同提取物HPLC指纹图谱与其补血作用的相关性,明确发挥补血作用的药效物质基础,并筛选出补血作用最好的提取方法。方法:采用HPLC法建立当归补血汤不同提取物的指纹图谱。皮下注射乙酰苯肼(APH)法复制血虚大鼠模型,观察4种提取物对血虚证大鼠血常规和血清造血因子的影响。通过正交偏最小二乘法(OPLS)将指纹图谱中各共有峰峰面积与补血作用综合药效数据相关联。结果:当归补血汤水提醇沉上清液提取物补血作用最好(P<0.01),4种提取物HPLC指纹图谱中共确定20个共有峰,有9个峰与综合药效呈高度正相关,其对补血作用的贡献大小顺序为峰14(芒柄花苷)、峰11(毛蕊异黄酮葡萄糖苷)、峰13(洋川芎内酯H),峰10(阿魏酸),峰8(未知),峰6(未知),峰9(绿原酸),峰12(洋川芎内酯I),峰18(美迪紫檀素)。结论:建立了当归补血汤的HPLC指纹图谱及其补血作用的谱效关系,并确定了补血作用的主要有效成分,为当归补血汤的药效物质基础研究和质量评价提供参考。

Objective:To investigate the correlation between the HPLC fingerprints and the hematopoietic effects of different extracts from Danggui Buxue Decoction(当归补血汤),to identify the active components responsible for the effects,and to determine the optimal extraction method.Methods:HPLC was utilized to establish the fingerprints of various extracts from Danggui Buxue Decoction.A rat model of blood deficiency was replicated using subcutaneous injections of acetylphenylhydrazine(APH).The impact of the four extracts on the blood routine and serum hematopoietic factors in rats with blood deficiency was observed.Orthogonal partial least squares(OPLS)was applied to correlate common peak areas in the fingerprints with the data on comprehensive pharmacological efficacy.Results:The supernatants of water extract-alcohol precipitation of Danggui Buxue Decoction exhibited the optimal hematopoietic effect(P<0.01),and twenty common peaks were identified in the HPLC fingerprints of the four extracts.Nine peaks showed a high positive correlation with the comprehensive efficacy,and their contributions to the hematopoietic effect,in a descending order,were peak 14(ononin),peak 11(calycosin-7-O-β-D-glucoside),peak 13(senkyunolide H),peak 10(ferulic acid),peak 8(unknown),peak 6(unknown),peak 9(chlorogenic acid),peak 12(senkyunolide I),and peak 18(methylnissolin).Conclusion:The spectrum-effect relationship and the HPLC fingerprint of Danggui Buxue Decoction for its hematopoietic effect were established,identifying the main active components for hematopoietic effect.This provided a reference for further pharmacodynamic substance research and quality evaluation of Danggui Buxue Decoction.