当归芍药散对果蝇生殖功能损伤模型的改善作用

Improvement Effect of Danggui ShaoYao San on the Reproductive Dysfunction Model in Drosophila Melanogaster

目的建立果蝇生殖功能损伤模型,观察当归芍药散(DSS)是否具有改善作用。方法利用雷公藤多苷构建果蝇生殖功能损伤模型,统计子代成虫数量、亲代超氧化物歧化酶(SOD)及过氧化氢酶(CAT)活性、亲代生殖相关基因的表达、F1代体质量、F1代发育相关基因,评价DSS药效。结果20 mg·mL^(-1)雷公藤多苷可显著抑制子代数(P<0.01),可用于建立果蝇生殖功能损伤模型。与模型对照组相比,中(10 mg·mL^(-1))、大(20 mg·mL^(-1))剂量DSS可显著增加果蝇子代成虫数量(P<0.01,P<0.05);大剂量DSS显著提高了果蝇亲代SOD及CAT活性(P<0.01);中、大剂量DSS可促进果蝇亲代生殖相关基因表达,其中中剂量DSS可使DEAD-Box解旋酶4(VASA)、生殖系α因子(Figla)mRNA表达增加(P<0.05,P<0.01),大剂量可使VASA、Figla和叉头盒转录因子(FoxO)mRNA表达增加(P<0.01);中剂量DSS组F1代雌性果蝇体质量显著提高(P<0.05),大剂量DSS组F1代雌、雄果蝇体质量均显著提高(P<0.05,P<0.01);中、大剂量DSS均可显著提高F1代发育相关基因哺乳动物雷帕霉素靶基因(mTOR)mRNA表达(P<0.01)。结论DSS能够改善雷公藤多苷诱导的果蝇生殖功能损伤。

Objective To establish a drosophila reproductive dysfunction model and observe whether Danggui Shaoyao San(DSS)has an improving effect.Methods Tripterygium glycosides were used to establish the drosophila reproductive dysfunction model.The number of offspring pupae,the parental superoxide dismutase(SOD)and catalase(CAT)activities,the parental reproductive-related gene expression,the F1 generation body weight,and the F1 generation development-related genes were measured to evaluate the efficacy of DSS.Results Tripterygium glycosides in a dose of 20 mg·mL^(-1)significantly inhibited the quantity of offspring(P<0.01)and were suitable for establishing the drosophila reproductive dysfunction model.Compared with the model control group,medium(10 mg·mL^(-1))and high(20 mg·mL^(-1))dose DSS significantly increased the number of offspring pupae(P<0.01,P<0.05).High-dose DSS significantly increased the SOD and CAT activities of the parent drosophila(P<0.01).Medium and high doses of DSS promoted the expression of drosophila parental reproduction-related genes,among which the medium dose DSS increased the mRNA expression of DEAD-Box helicase 4(VASA)and factor in the germline alpha(Figla)(P<0.05,P<0.01),and the high dose DSS increased the mRNA expression of VASA,Figla and forkhead box O(FoxO)(P<0.01).The medium dose of DSS significantly increased the body weight of F1-generation female drosophila(P<0.05),and the high dose of DSS significantly increased the body weight of both F1-generation female and male drosophila(P<0.05,P<0.01).Both the medium and high dose DSS increased the mRNA expression of the F1 generation development-related gene mammalian target protein of rapamycin(mTOR)(P<0.01).Conclusions DSS improves the reproductive function injury of drosophila induced by tripterygium glycosides.