大豆磷脂酶D家族基因全基因组鉴定及耐盐性分析

Genome-wide Identification and Salt Tolerance Analysis of Phospholipase D Gene Family in Soybean

磷脂酶D(Phospholipase D,PLD)及其产物磷脂酸(Phosphatidic Acid,PA)在植物生长发育和胁迫响应的调控中起着重要作用。目前,对大豆PLD基因家族的研究仍不够全面。为进一步寻找与大豆抗逆性相关的潜在候选基因,本研究利用全基因组分析方法对28个GmPLDs的基因结构、蛋白保守结构域、共线性和系统发育关系等进行研究,并采用qRT-PCR方法分析基因在盐胁迫下的表达量。结果表明:大豆PLD家族基因分布在16条染色体上,片段复制在大豆PLD基因家族的扩展中发挥了重要作用。基因表达模式分析显示GmPLDs在不同组织中的表达存在差异,启动子顺式作用元件分析显示GmPLDs具有参与非生物胁迫和激素途径的潜在功能。qRT-PCR分析显示,18个大豆PLDs基因表达受盐胁迫诱导显著上调,可能参与大豆耐盐胁迫。研究结果为解析及克隆大豆耐盐基因提供理论依据,并为挖掘响应非生物逆境胁迫GmPLDs基因提供了基础信息。

Phospholipase D(PLD)and its product phosphatidic acid(PA)play important roles in the regulation of plant growth and development and stress response.Currently,the study of the PLD gene family in soybean is still not comprehensive enough.In order to further search for potential candidate genes related to soybean stress resistance,we used genome-wide analysis to study the gene structure,protein conserved domains,collinearity and phylogenetic relationships of 28 GmPLDs.And the expression levels of genes under salt stress were analyzed by qRT-PCR.The results indicated that GmPLDs were distributed on 16 chromosomes,fragment duplication played an important role in the expansion of the soybean PLD gene family.The gene expression pattern analysis showed that the expression of GmPLDs varied in different tissues,and the promoter cis-acting element analysis showed that GmPLDs have potential functions involved in abiotic stresses and hormonal pathways.The qRT-PCR Analysis showed that 18 GmPLDs expression was significantly up-regulated by salt stress induction,which may be involved in soybean salt tolerance stress.The results provide theoretical basis for the analysis and cloning of soybean salt-tolerant genes,and provide basic information for mining GmPLDs in response to abiotic stress.