河南省18家血站实验室丙氨酸氨基转移酶检测不合格情况分析

Status analysis on positive ALT detection in 18 blood station labs of Henan Province

目的通过分析河南省18家血站实验室丙氨酸氨基转移酶(alanine aminotransferase,ALT)检测数据,探索不同实验室间不合格率存在差异的影响因素,提升河南省血站实验室室间检测水平,推动血站规范化建设。方法以河南省18家血站实验室2017—2019年无偿献血者ALT检测结果为调查对象,收集每年检测总数、不合格数、检测设备、检测试剂等资料,使用SPSS统计学软件对结果进行分析。结果河南省18家血站实验室ALT不合格率为1.02‰~12.76‰,差异有统计学意义(χ^(2)=9049.45,P<0.05);初筛不同设备及试剂不合格率为2.98‰~6.78‰,差异有统计学意义(χ^(2)=927.04,P<0.05);使用不同复检设备不合格率为1.18‰~11.46‰,差异有统计学意义(χ^(2)=4978.83,P<0.05);使用不同复检试剂不合格率为1.02‰~11.46‰,差异有统计学意义(χ^(2)=6027.75,P<0.05)。结论通过比对各实验室检测设备、检测试剂等影响因素,为逐步降低因ALT检测的血液报废率,提高血站实验室检测水平同质化,稳步推进河南省血站规范化建设提供参考。

Objective To understand the alanine aminotransferase(ALT)detection of 18 blood station laboratories(labs)in Henan province,explore the influencing factors of the difference in the unqualified rate among different labs,improve the level of inter-laboratory testing in Henan province,and promote the standardized construction of blood stations.Methods The ALT test results of voluntary blood donors from 18 blood stations in Henan province during 2017-2019 were selected.The annual total number of tests,unqualified numbers,test equipment,test reagents and other data were collected,and the results were analyzed by SPSS.Results The ALT unqualified rate of 18 blood stations in Henan province was 1.02‰-12.76‰,which was significant differences(χ^(2)=9049.45,P<0.05).The unqualified rate of different equipment and reagents in primary screening was 2.98‰-6.78‰,and the difference was significant(χ^(2)=927.04,P<0.05).The unqualified rate of different retest equipment was 1.18‰-11.46‰,which was significant difference(χ^(2)=4978.83,P<0.05).The unqualified rate of different retest reagents was 1.02‰-11.46‰,and the difference was significant(χ^(2)=6027.75,P<0.05).Conclusion By comparing the lab test equipment,reagents and other influencing factors,we can gradually reduce the blood scrap rate due to ALT,improve the homogenization of lab test level in blood stations,and steadily promote the standardized construction of blood stations in Henan province.