内生真菌联合接种对雷公藤生长及养分含量的影响

Effects of Combined Inoculation of Endophytic Fungi on the Growth and Nutrient Content of Tripterygium wilfordii

为探究雷公藤内生真菌联合接种对宿主植物生长及养分含量的影响,将4株雷公藤内生真菌NS6、NS31、NS32和NS33成对组合接种于雷公藤组培苗上,以无菌块的PDA培养基作为CK,测定组培苗的生长、叶绿素含量及C、N、P、K养分含量。结果表明:联合接种内生真菌显著促进了雷公藤组培苗的株高,其中菌株NS6+NS33和NS6+NS32接种处理分别是CK的2.72和2.28倍;菌株NS6+NS32联合接种的根长和干质量也显著高于CK;菌株NS6+NS33接种的干质量显著高于CK。与单独接种相比,菌株NS6+NS32联合接种对组培苗的质量增加具有协同效应。此外,菌株NS6+NS32和NS6+NS33组合显著提高了叶绿素a、b及a+b含量。各菌株的联合接种相比CK增加了C、N含量,但对P和K的影响不显著。内生真菌对雷公藤组培苗生长及养分含量的影响因不同真菌种类组合而异,NS6+NS32联合接种的生长促进效果更好。

To investigate the effects of combined inoculation of endophytic fungi on the growth and nutrient content of host plants,4 strains of endophytic fungi,Penicillium steckii NS6,Meyerozyma guilliermondii NS31,Hypocrea nigricans NS32 and Fusarium oxysporum NS33,were paired and inoculated into micropropagated Tripterygium wilfordii seedlings.Sterile PDA medium plugs were served as controls.The growth,chlorophyll content and nutrient content(C,N,P and K)of the micropropagated seedlings were measured.The results indicated that combined inoculation of endophytic fungi significantly enhanced the height of micropropagated seedlings.Specially,the height under NS6+NS33 and NS6+NS32 inoculation was 2.72 and 2.28 times greater than the control,respectively.Additionally,the root length and dry mass under NS6+NS32 inoculation were significantly higher compared to the control.The dry mass under NS6+NS33 inoculation was significantly higher compared to the control.The combined inoculation of NS6+NS32 had synergistic effects on increasing the mass of the micropropagated seedlings,surpassing the effects of single inoculation.Furthermore,inoculation of NS6+NS32 and NS6+NS33 significantly elevated chlorophyll a,b,and a+b levels.The combined inoculation of each fungal strain increased the C and N content compared to the control.However,there was no significant effect on P and K levels.The influence of endophytic fungi on the growth and nutrient content of micropropagated T.wilfordii seedlings varied depending on the combination of fungal species.Notably,the combination of NS6 and NS32 strains exhibited enhanced growth promotion effects.