应用单细胞转录组测序技术揭示慢性乙型肝炎免疫学特征的研究进展

Revealing research progress for immunological characteristics of chronic hepatitis B applying single-cell RNA sequencing technology

慢性乙型肝炎(CHB)是全球主要的公共卫生问题之一。乙型肝炎病毒感染的潜在免疫机制尚未研究清楚。单细胞转录组测序(scRNA-seq)技术作为近年来新兴的一种测序方法,可以在单细胞水平上提供全转录组的高通量视图,揭示CHB病程中免疫细胞转录谱的精细变化,为探究CHB的免疫发病机制、探索潜在治疗靶点提供了技术支持。现对scRNA-seq技术在CHB免疫学特征的研究进展进行总结,为治疗CHB提供新思路。

Chronic hepatitis B(CHB)is one of the major public health problems worldwide.The potential immunological mechanisms of hepatitis B virus(HBV)infection has not yet been clearly studied.Single-cell RNA sequencing(scRNA-seq)technology,as an emerging sequencing method in recent years,can provide a high-throughput view of the whole transcriptome at the single-cell level,reveal subtle changes in transcriptional profiles of immune cells during the course of chronic hepatitis B,and provide technological support for exploring the immunopathogenesis and potential therapeutic targets for CHB.This article summarizes the research progress conducted on scRNA-seq technology in order to provide novel therapeutic ideas for the immunological characteristics of chronic hepatitis B.