菊花扦插生根剂的筛选及综合评价

Screening Research and Comprehensive Evaluation of Rooting Agents for Chrysanthemum Cuttings

为筛选出在贫瘠红壤条件下对菊花扦插生根有较好促进作用的生根剂,以红壤土为基质,采用不同的生根剂对菊花插穗进行处理,对其生根率、生根时间、根长、根数、根干质量、根粗等表型指标进行测定,以主成分分析评价生根剂的效果。对促生效果较好的生根剂处理的菊花扦插苗进行营养物质、激素含量和酶活性等测定以探究其促生机理,以极差分析法明确影响菊花扦插生根的主要生理指标。主成分分析将生根表型的7个单项指标转换成2个相互独立的综合指标,累计贡献率为89.69%。由扦插苗的生根效果综合得分可知,那氏齐齐发诱导剂得分最高,与对照相比,生根时间提前了2 d,15 d时生根率提高了34.44%,30 d时的生根数增加97.80%,根干质量增加54.97%,根粗增加24.24%;双吉尔–GGR6号得分次之,相较对照根长增加了78.64%。在扦插苗的各项生理指标中,IAA极差值最大,是影响菊花扦插生根的关键因素;可溶性蛋白含量是影响菊花扦插生根的最重要营养物质。综上,那氏齐齐发诱导剂和双吉尔–GGR6号促生根作用较好,可用作促菊花扦插的优良生根剂。

In order to screen out the rooting agent that has a good effect on the rooting of chrysanthemum cuttings under the condition of barren red soil,the red soil was used as the substrate,and different rooting agents were used to treat the chrysanthemum cuttings,and the phenotypic indexes such as rooting rate,rooting time,root length,root number,root dry weight,and root thickness were determined.The effect of rooting agent was evaluated by principal component analysis.In order to explore the mechanism of growth-promoting seedlings of chrysanthemum cuttings treated with rooting agent,nutrients,hormone content and enzyme activity were determined,and the main physiological indexes affecting the rooting of chrysanthemum cuttings were clarified by extreme analysis.Principal component analysis(PCA)converted 7 individual indexes of rooting phenotype into 2 independent composite indexes,with a cumulative contribution rate of 89.69%.According to the comprehensive score of rooting of chrysanthemum cuttings,Nashi 778 has the highest score.Compared with the control,the rooting time was advanced by 2 days,the rooting rate increased by 34.44%on the 15th day,the number of roots at the 30th day increased by 97.80%,the root dry weight increased by 54.97%,and the root thickness increased by 24.24%.The second score of Double Gil GGR-6 was 78.64%higher than that of the control in root length.Among the physiological indexes of cuttings,the IAA range was the largest,which was the key factor affecting the rooting of chrysanthemum cuttings.Soluble protein content is the most important nutrient affecting the rooting of chrysanthemum cuttings.In conclusion,Nashi 778 inducer and Double Gil GGR-6 had good root-promoting effects,and could be used as excellent rooting agents for promoting chrysanthemum cuttings.