颞下颌关节滑液中IL-1β、IL-18、NLRP3以及Caspase-1水平检测在颌骨成骨细胞焦亡中的意义

Significance of detection of IL-1β,IL-18,NLRP3 and Caspase-1 in synovial fluid of temporomandibular joint in pyroptosis of jawbone osteoblasts

目的探讨颞下颌关节滑液中白细胞介素(IL)-1β、IL-18、NOD样受体热蛋白结构域相关蛋白(NLRP3)以及半胱氨酸蛋白酶-1(Caspase-1)水平检测在颌骨成骨细胞焦亡中的意义。方法选取2020年8月至2021年8月牡丹江医学院第二附属医院收治的颞下颌关节紊乱病患者64例作为研究对象。所有患者均进行电子计算机断层扫描(CT)或核磁共振成像(MRI)扫描,根据检查结果,将伴有髁突骨质破坏的28例患者作为研究组,不伴有髁突骨质破坏的36例患者作为对照组。比较两组患者焦亡经典途径相关分子的表达水平,观察两组患者颞下颌关节滑液中成骨细胞72 h内的焦亡情况,分析检测IL-1β、IL-18、NLRP3以及Caspase-1水平对颌骨成骨细胞焦亡的临床价值。结果研究组IL-1β、IL-18、NLRP3以及Caspase-1水平均明显高于对照组,差异有统计学意义(P<0.05)。研究组24、48和72 h颞下颌关节滑液中成骨细胞Caspase-1+PI+水平均明显高于对照组,差异有统计学意义(P<0.05)。结论IL-1β、IL-18、NLRP3以及Caspase-1水平在骨质破坏患者中均明显升高。

Objective To explore the significance of interleukin(IL)-1β,IL-18,NOD-like receptor thermal protein domain associated protein 3(NLRP3)and cysteinyl aspartate specific proteinase-1(Caspase-1)in temporomandibular joint synovial fluid in the focal death of jaw osteoblasts.Methods A total of 64 patients with temporomandibular joint disorder admitted to the Affiliated Second Hospital Mudanjiang Medical University from August 2020 to August 2021 were selected as study subjects.All patients were examined by computed tomography(CT)or magnetic resonance imaging(MRI).According to the examination results,28 patients with condylar bone destruction were selected as the study group,and 36 patients without condylar bone destruction were selected as the control group.The expression levels of molecules related to the classical pyroptosis pathway were compared between the two groups,and the pyroptosis of osteoblasts in the synovium of temporomandibular joint of the two groups were observed within 72 h,and the clinical value of detecting IL-1β,IL-18,NLRP3 and Caspase-1 levels on pyroptosis of mandibular osteoblasts was analyzed.Results The levels of IL-1β,IL-18,NLRP3 and Caspase-1 in the study group were significantly higher than those in the control group,with statistical significance(P<0.05).The Caspase-1+PI+levels of osteoblasts in synovial fluid of temporomandibular joint in the study group were higher than those in the control group at 24,48 and 72 h,with statistical significance(P<0.05).Conclusion The levels of IL-1β,IL-18,NLRP3 and Caspase-1 are all significantly increased in patients with bone destruction.