野菊花不同溶剂提取物的体外抗氧化活性研究

In Vitro Antioxidant Activity of Extracts from Chrysanthemum Indicum Flos in Different Solvents

目的 探讨野菊花不同溶剂提取物的体外抗氧化活性,并采用硝酸铝显色法测定其总黄酮含量。方法 检测石油醚、乙酸乙酯、正丁醇、残渣萃取、原浸膏提取物对1,1-二苯-2-苦基肼(DPPH)自由基和2,2′-联氨-(3-乙基苯并噻唑啉-6-磺酸)二氨盐(ABTS)自由基的清除能力,以评价其不同溶剂提取物的抗氧化活性;以芦丁为对照品,采用硝酸铝显色法测定各提取物的总黄酮含量。结果 野菊花上述5种溶剂提取物清除DPPH自由基的半数抑制浓度(IC_(50))分别为(312.2±10.29)μg/mL、(3.21±0.05)μg/mL、(40.60±1.73)μg/mL、(254.85±3.65)μg/mL、(114.57±3.75)μg/mL,清除ABTS自由基的IC_(50)分别为(112.05±2.00)μg/mL、(19.56±0.92)μg/mL、(24.03±0.77)μg/mL、(88.13±3.26)μg/mL、(53.78±1.19)μg/mL。野菊花上述5种溶剂提取物的总黄酮含量分别为(14.08±0.42)%,(42.43±1.63)%,(18.13±0.58)%,(5.17±0.085)%,(16.26±0.71)%。结论 野菊花不同溶剂提取物均有很强的抗氧化活性,其中乙酸乙酯提取物的抗氧化活性最强,且乙酸乙酯提取物的总黄酮含量也最高,野菊花中的黄酮类成分可能是其抗氧化的主要活性成分。该研究为从野菊花中提取天然抗氧化剂研究提供了参考。

Objective To investigate the in vitro antioxidant activity of extracts from Chrysanthemum Indicum Flos in different solvents,and to determine the content of total flavonoids by the aluminum nitrate colorimetric method.Methods The scavenging abilities of petroleum ether,ethyl acetate,n-butanol,residue extraction,and original extract on 1,1-diphenyl-2-picrylhydrazine(DPPH)free radicals and 2,2′-hydrazine-(3-ethylbenzothiazoline-6-sulfonic acid)diamine salt(ABTS)free radicals were detected to evaluate the antioxidant activity of extracts from Chrysanthemum Indicum Flos in different solvents.With rutin as a reference substance,the content of the total flavonoids of each extract was determined by the aluminum nitrate colorimetric method.Results The half maximal inhibitory concentrations(IC_(50))of the extracts from Chrysanthemum Indicum Flos in the above five solvents for scavenging DPPH free radicals were(312.2±10.29)μg/mL,(3.21±0.05)μg/mL,(40.60±1.73)μg/mL,(254.85±3.65)μg/mL,and(114.57±3.75)μg/mL,respectively;the IC_(50) values for clearing ABTS free radicals were(112.05±2.00)μg/mL,(19.56±0.92)μg/mL,(24.03±0.77)μg/mL,(88.13±3.26)μg/mL,and(53.78±1.19)μg/mL,respectively.The contents of total flavonoids of the extracts from Chrysanthemum Indicum Flos in the above five solvents were(14.08±0.42)%,(42.43±1.63)%,(18.13±0.58)%,(5.17±0.085)%,and(16.26±0.71)%,respectively.Conclusion Extracts from Chrysanthemum Indicum Flos in different solvents have strong antioxidant activity,among which ethyl acetate extract has the strongest antioxidant activity,and the content of total flavonoids of ethyl acetate extract is also the highest.Flavonoids in Chrysanthemum Indicum Flos may be the main active ingredients with antioxidant activity.This study provides a reference for the extraction of natural antioxidants from Chrysanthemum Indicum Flos.