SYT7调控Hedgehog/Gli信号通路对胶质母细胞瘤进展的影响

Effect of SYT7 regulation of Hedgehog/Gli signaling pathway on the progression of glioblastoma

目的探讨人突触结合蛋白Ⅶ(synaptotagmin-7,SYT7)通过调控Hedgehog/Gli信号通路对胶质母细胞瘤进展的影响。方法通过构建并转染短发夹RNA(short hairpin RNA,shRNA)慢病毒载体,利用定量反转录聚合酶链反应(quantitative reverse transcriptase-medi ated polymerase chain reaction,qRT-PCR)分析SYT7在人脑胶质瘤U87细胞系中的表达,采用蛋白质印迹和qRT-PCR分析U87细胞中SYT7 shRNA的敲低效率。通过细胞计数、细胞周期分析和动物实验验证SYT7在胶质母细胞瘤中的作用。结果qRT-PCR分析显示SYT7在U87细胞系中的表达高于正常脑细胞系。蛋白质印迹和qRT-PCR分析证实SYT7 shRNA在U87细胞中的有效敲低。细胞计数显示SYT7 shRNA的表达通过慢病毒载体下调SYT7,抑制U87细胞的生长;流式细胞术证实细胞周期阻滞。此外,基因富集分析和蛋白质印迹发现SYT7通过促进p53磷酸化与Hedgehog/Gli信号通路相关,进而在胶质母细胞瘤中发挥作用。结论SYT7通过调控Hedgehog/Gli信号通路促进胶质母细胞瘤的生长,因此SYT7可能是治疗胶质瘤的潜在靶点。

Objective To investigate the effect of synaptotagmin-7(SYT7)on the progression of glioblastoma by regulating Hedgehog/Gli signaling pathway.Methods The expression of SYT7 in U87 cell line was analyzed by quantitative reverse transcriptase-mediated polymerase chain reaction(qRT-PCR)and transfection with short hairpin RNA(shRNA)lentiviral vector.Western blotting and qRT-PCR were used to analyze the knockdown efficiency of SYT7 shRNA in U87 cells.Cell counting,cell cycle analysis,and animal studies confirm the role in glioblastoma.Results qRT-PCR analysis showed that the expression of SYT7 in U87 cell line was higher than human normal glial cell line.Western blotting and qRT-PCR analysis showed low knockdown efficiency of SYT7 shRNA in U87 cells.Cell counts showed that the lentiviral vector expressing SYT7 shRNA down-regulated SYT7 and inhibited the growth of U87 cells.Flow cytometry confirmed cell cycle block,gene enrichment analysis and Western blotting revealed that SYT7 was associated with Hedgehog/Gli signaling pathway by promoting p53 phosphorylation.It was further demonstrated in glioblastoma by animal experiments.Conclusion The results of this study suggest that SYT7 promotes glioblastoma growth by regulating the Hedgehog/Gli signaling pathway,so SYT7 may be a potential therapeutic target for glioma.