不同中医证型特应性皮炎患者外周血Th1、Th2、Th17细胞及miR-155表达分析

Analysis of Th1,Th2,Th17cells and miR-155expression in peripheral blood of patients with atopic dermatitis with different TCM syndromes

目的:分析研究不同中医证型特应性皮炎(AD)患者外周血辅助性T细胞1(Th1)、Th2、Th17细胞及微小RNA-155(miR-155)表达。方法:回顾性选取2021年6月~2022年6月我院收治的AD患者作为AD组(n=112),根据不同中医证型将患者分为脾虚湿蕴组(n=46)、湿热内蕴组(n=37)和血虚风燥组(n=29),另选60例本院同期体检健康人群为对照组(n=60)。比较各组外周血Th1、Th2、Th17细胞百分比差异、Th1类细胞因子(IFN-γ)、Th2类细胞因子(IL-4)、Th17类细胞因子(IL-17)及miR-155表达。结果:AD组外周血Th1、IFN-γ水平低于对照组,Th2、Th17、IL-4、IL-17、miR-155表达均高于对照组(P<0.05);不同中医证型的AD患者外周血Th1、IFN-γ水平比较差异无统计学意义(P>0.05);不同中医证型的Th2、Th17、IL-4、IL-17表达水平相比,湿热内蕴组>脾虚湿蕴组>血虚风燥组(P<0.05);不同中医证型的miR-155表达水平相比,湿热内蕴组>脾虚湿蕴组>血虚风燥组(P<0.05)。结论:不同中医证型AD患者外周血Th2、Th17细胞及相关细胞因子与miR-155表达存在差异性,检测其表达水平有助于指导AD中医辨证分型和治疗。

Objective:To analyze the expression of peripheral blood helper T cells 1(Th1),Th2,Th17and microRNA-155(miR-155)in patients with atopic dermatitis(AD)with different TCM syndromes.Methods:AD patients admitted to our hospital from June 2021to June 2022were retrospectively selected as AD group(n=112).According to different TCM syndrome types,the patients were divided into spleen deficiency and dampness group(n=46),damp-heat intrinsic group(n=37)and blood deficiency wind-dryness group(n=29).Another 60healthy people in our hospital during the same period were selected as control group(n=60).The percentage difference of Th1,Th2and Th17cells,the expression of Th1cytokines(IFN-γ),Th2cytokines(IL-4),Th17cytokines(IL-17)and miR-155in peripheral blood of all groups were compared.Results:The levels of Th1and IFN-γin peripheral blood of AD group were lower than those of control group,and the expressions of Th2,Th17,IL-4,IL-17and miR-155were higher than those of control group(P<0.05).There was no significant difference in Th1and IFN-γlevels in peripheral blood of AD patients with different TCM syndrome types(P>0.05).The expression levels of Th2,Th17,IL-4and IL-17in different TCM syndrome types were as follows,with damp-heat intrinsic group>spleen deficiency and dampness group>blood deficiency wind-dryness group(P<0.05).The expression levels of miR-155in different TCM syndrome types were as follows,with damp-heat intrinsic group>spleen deficiency and dampness group>blood deficiency wind-dryness group(P<0.05).Conclusion:There are differences in the expression of Th2,Th17cells and related cytokines and miR-155in peripheral blood of AD patients with different TCM syndrome types.Detection of their expression levels is helpful to guide the TCM syndrome differentiation and treatment of AD.