长链非编码RNA RNF157-AS1对肺腺癌细胞恶性生物学行为的影响

Effects of long non-coding RNA RNF157-AS1 on malignant biological behavior of lung adenocarcinoma cells

目的通过细胞实验探究长链非编码RNA RNF157-AS1对肺腺癌细胞恶性生物学行为的影响。方法利用实时荧光定量PCR(qRT-PCR)检测RNF157-AS1在肺腺癌细胞株(H1299、A549、PC9和Calu3)和正常肺上皮细胞(HBE)中的表达;构建小干扰RNA转染至肺腺癌细胞中敲降RNF157-AS1的表达,利用实时荧光定量PCR(qRT-PCR)验证干扰效率;在筛选的两种肺腺癌细胞株中成功干扰RNF157-AS1后,分为对照组(NC组)及实验组(si1组,si2组),分别利用CCK8实验、Transwell迁移实验和Matrigel侵袭实验探究RNF157-AS1对肺腺癌细胞增殖、迁移和侵袭能力的影响。结果相较于HBE,RNF157-AS1在4种肺腺癌细胞株中的表达显著上调(均P<0.01),将其中表达量最高H1299和PC9细胞株为后续试验细胞株;经qRT-PCR证实,2种小干扰RNA均能有效敲降RNF157-AS1的表达,干扰效率在70%以上(均P<0.01);CCK8实验表明,与对照组相比,干扰RNF157-AS1的表达后,细胞的增殖能力明显下降(均P<0.01);Transwell实验表明,与对照组相比,干扰RNF157-AS1的表达后,细胞的迁移能力明显下降(P<0.01);Matrigel实验表明,与对照组相比,干扰RNF157-AS1后,细胞的侵袭能力明显下降(均P<0.01)。结论RNF157-AS1在肺腺癌细胞中高表达,并且影响肺腺癌细胞的增殖、迁移、侵袭能力。

Objective To investigate the effects of long non-coding RNA RNF157-AS1 on the malignant biological behavior of lung adenocarcinoma cells through cell experiments.Methods Quantitative Real-time fluorescent PCR(qRT-PCR)was used to detect the expression of RNF157-AS1 in lung adenocarcinoma cell lines(H1299,A549,PC9 and Calu3)and normal lung epithelial cells(HBE).Small interfering RNA was transfected into lung adenocarcinoma cells to knock down the expression of RNF157-AS1,and the interfering efficiency was verified by qRT-PCR.After successfully interfering with RNF157-AS1 in two lung adenocarcinoma cell lines,they were divided into control group(NC group)and interference group(si1 group,si2 group).The effects of RNF157-AS1 on the proliferation,migration and invasion of lung adenocarcinoma cells were investigated by CCK8 assay,Transwell migration assay and Matrigel invasion assay,respectively.Results Compared with HBE,the expression of RNF157-AS1 in 4 lung adenocarcinoma cell lines was significantly up-regulated(P<0.01 for all),and H1299 and PC9 cell lines with the highest expression levels were selected as the follow-up test cell lines.qRT-PCR confirmed that the two small interfering RNAs could effectively knock down the expression of RNF157-AS1,and the interference efficiency was more than 70%(P<0.01).CCK8 assay showed that,compared with the control group,the proliferation ability of cells was significantly decreased after interference of RNF157-AS1 expression(P<0.01).The Transwell assay showed that,compared with the control group,the migration ability of RNF157-AS1 cells was significantly decreased after interference(P<0.01).Matrigel′s experiment showed that interference with RNF157-AS1 significantly reduced the invasive ability of cells compared with the control group(P<0.01).Conclusion RNF157-AS1 is highly expressed in lung adenocarcinoma cells,and affects the proliferation,migration and invasion of lung adenocarcinoma cells.